Phenotyping of intrahepatic and peripheral blood lymphocytes in patients with chronic hepatitis C

Digestive Diseases and Sciences
A TranS Benzaken

Abstract

The host immune responses have been suggested to play a role in liver injury occurring in patients with chronic hepatitis C. In order to explore the relationship between the relative proportions of intrahepatic and peripheral blood lymphocytes (IHL, PBL), the levels of viremia, and the histological hepatitis activity score, three-color fluorescence-activated cytometric analysis was performed for 36 patients with chronic hepatitis C and six control subjects without chronic hepatitis. The liver biopsy was performed before any antiviral therapy. Each liver specimen was divided into two parts: one for histological examination and one for immunological analysis. Tricolor CD45 was used to improve "lymphogating." Fluorescein isothiocyanate- or phycoerythrin-conjugated monoclonal antibodies with specificity for CD3, CD4, CD8, and CD20 (lymphocyte subpopulations), for CD69 (activated lymphocytes), and for CD16/56 (natural killer cells) were used. The livers of patients with chronic hepatitis C contained a greater proportion of CD4+ lymphocytes that exhibited marked expression of CD69 than in control subjects (20.7 +/- 7.3% vs 10.2 +/- 4.6%, P = 0.027). Moreover, in patients with chronic hepatitis C, the proportion of CD4+ IHL correlated...Continue Reading

Citations

May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J E SchmitzN L Letvin
Mar 26, 2011·European Journal of Applied Physiology·Lara A CarlsonR J Leclair
Jun 10, 2004·World Journal of Gastroenterology : WJG·Qiao-Ling Sun, Wei Ran
Dec 25, 2010·Journal of Biochemical and Molecular Toxicology·Maged BarakatJackleen Raafat
Dec 25, 2019·Cold Spring Harbor Perspectives in Medicine·Hugo R Rosen, Lucy Golden-Mason

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