Phenyl-modified reversed-phase liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry: a universal method for the analysis of partially oxidized aromatic hydrocarbons

Analytical Chemistry
Thomas LetzelReinhard Niessner

Abstract

A new liquid chromatographic method for the efficient separation of aromatic compounds having a wide range of sizes, molecular structures, and polarities has been developed. Based on a phenyl-modified silica reversed stationary phase and a methanol-water solvent gradient, it allows the separation of mono- and polycyclic aromatic hydrocarbons (PAHs) having up to five condensed aromatic rings and partially oxidized derivatives within a single chromatographic run of 40-min duration. The applicability of the method is demonstrated using 81 reference substances (PAHs, phenols, quinones, acids, lactones, esters, etc.) and real samples of environmental, medical, and technical relevance (ozonized PAHs, lake water, human urine, diesel exhaust condensates). The retention times of the investigated aromatics exhibit a regular increase with molecular mass and a systematic decrease with increasing number and polarity of functional groups. In case of intramolecular hydrogen bonding, a positive shift of retention time provides additional structural information. The combination of chromatographic retention time with the molecular mass and structural information from mass spectrometric detection allows the tentative identification of unknown aro...Continue Reading

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Citations

Jan 19, 2010·Analytical and Bioanalytical Chemistry·Carine JacquesLaurent Debrauwer
Sep 6, 2013·Analytical and Bioanalytical Chemistry·Steven G O'ConnellKim A Anderson
Jan 9, 2008·Archives of Environmental Contamination and Toxicology·Kentaro MisakiTomonari Matsuda
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Jing ZhangYinsheng Wang
Mar 20, 2010·Journal of the American Society for Mass Spectrometry·Mahmoud BatainehWerner Brack
Aug 20, 2002·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·Ulrich Pöschl
Jan 19, 2013·AMB Express·Alexander KarichMartin Hofrichter
Jul 27, 2001·Journal of Mass Spectrometry : JMS
Sep 14, 2010·Journal of Environmental Quality·Benjamin A Musa Bandowe, Wolfgang Wilcke
Oct 30, 2008·Journal of Agricultural and Food Chemistry·Bradley W Bolling, Kirk L Parkin
Jul 25, 2021·The Science of the Total Environment·Manuel Méndez García, Martha Patricia García de Llasera
Jul 18, 2006·Analytical Chemistry·Chris A JakoberPeter G Green

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