PMID: 3756210Oct 1, 1986Paper

Phenylalanine ammonia-lyase immobilized in microcapsules for the depletion of phenylalanine in plasma in phenylketonuric rat model

Biochimica Et Biophysica Acta
L Bourget, T M Chang

Abstract

Microencapsulation of the enzyme phenylalanine ammonia-lyase was developed for in vivo depletion of systemic phenylalanine in phenylketonuric rats. Compared to normal rats, systemic phenylalanine blood levels in phenylketonuric rats was increased by 15-20-fold. Daily oral administration of 1 unit of phenylalanine ammonia-lyase-loaded artificial cells to phenylketonuric rats lowered the systemic phenylalanine level to 58% +/- 18% (mean + S.D.) in 7 days (P less than 0.010), while 5 units lowered the systemic phenylalanine level to 25% +/- 8%. 5 units of the immobilized enzyme lowered the systemic phenylalanine level to normal levels within 6 days. Phenylketonuric treated rats showed no signs of abnormal behavior and weight loss compared to phenylketonuric non-treated rats. The immobilized enzyme within artificial cells is therefore protected against low gastrointestinal pH and proteolytic enzymes.

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Citations

Aug 5, 1998·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·T M Chang
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·H L Levy
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·C N SarkissianC R Scriver
Jun 1, 1988·Artificial Organs·T M ChangG Reach
Aug 1, 2012·Drug Delivery and Translational Research·Jaspreet Singh KochharLifeng Kang
May 22, 2013·Critical Reviews in Biotechnology·Jian Dong CuiZhi Lei Tan
Feb 3, 2015·Critical Reviews in Biotechnology·Valeria E BosioGuillermo R Castro
Mar 29, 2014·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Fan Wu, Cheemeng Tan
Mar 30, 2004·Artificial Organs·Thomas Ming Swi Chang
Jan 1, 1995·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·S Safos, T M Chang
Jan 1, 1993·Biomaterials, Artificial Cells, and Immobilization Biotechnology : Official Journal of the International Society for Artificial Cells and Immobilization Biotechnology·T M Chang
Jan 1, 1992·Biomaterials, Artificial Cells, and Immobilization Biotechnology : Official Journal of the International Society for Artificial Cells and Immobilization Biotechnology·T M Chang
Jan 1, 1987·Biomaterials, Artificial Cells, and Artificial Organs·T M Chang
Aug 6, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Woomi KimReuben Matalon
Aug 1, 1988·Acta Paediatrica Japonica; Overseas Edition·C R Scriver
Jul 1, 1988·Journal of Biomaterials Applications·T M Chang
Mar 2, 2005·Nature Reviews. Drug Discovery·Thomas Ming Swi Chang
Apr 2, 1999·Nutrition Reviews·O de FreitasL J Greene
May 20, 2003·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Thomas Ming Swi Chang
Sep 1, 1993·Cell Transplantation·S E Raper, J M Wilson
Mar 19, 2004·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Thomas Ming Swi Chang
Sep 17, 2005·Molecular Genetics and Metabolism·Christineh N Sarkissian, Alejandra Gámez

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