Phenylalanine hydroxylase expression in primary rat hepatocytes is modulated by oxygen concentration

Molecular Genetics and Metabolism
Ming YingAurora Martinez

Abstract

In this work we have investigated the regulation of rat phenylalanine hydroxylase (rPAH) expression by oxygen in primary cultures of rat hepatocytes. We show that rPAH is negatively modulated at the mRNA, protein and activity levels by pO(2) of 16% (periportal hepatic levels) compared to 8% (perivenous hepatic levels). Our results suggest that PAH might be metabolically zonated in vivo, and preferentially found in perivenous hepatocytes with high glucose consumption and largely influenced by insulin levels.

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Citations

Apr 5, 2016·Ageing Research Reviews·Brett R Ipson, Alfred L Fisher
Jan 1, 2012·Metabolites·Mehmet A OrmanMarianthi G Ierapetritou
Jun 20, 2021·Life Science Alliance·Angel Loza-ValdesGrzegorz Sumara

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