Phenylalanine utilization by the gut and liver measured with intravenous and intragastric tracers in pigs

The American Journal of Physiology
Barbara StollPeter J Reeds

Abstract

To investigate intestinal and hepatic metabolism of phenylalanine, four conscious pigs (7.5 kg), bearing arterial, venous, and hepatic portal catheters, were fasted for 12 h and infused with [phenyl-2H5]phenylalanine via a peripheral vein and [carboxyl-13C]phenylalanine via the stomach. During the first 6 h of the infusion, the pigs remained fasted and received only the intravenous tracer. During the second 6 h, they received an intragastric infusion of milk replacer and both tracers. In the fasted state, the portal-drained viscera extracted 10% (P < 0.025) of the arterial [2H5]phenylalanine flow of the pigs. In the fed state, the splanchnic tissues metabolized 45% of the enteral tracer and intestinal metabolism accounted for 76% of the total splanchnic extraction. The tracer-to-tracee ratio of both tracers in apolipoprotein B-100 (apo B-100) phenylalanine was twofold (P < 0.001) higher than that of hepatic free phenylalanine. The ratios of the two tracers in portal (13C/2H; 1.66) and apo B-100 (1.76) phenylalanine were similar but higher (P < 0.05) than that of arterial phenylalanine (1.29). We conclude that intestinal metabolism dominates the splanchnic extraction of enteral phenylalanine and that in the fed state, the hepati...Continue Reading

References

Apr 15, 1976·The Biochemical Journal·E B Fern, P J Garlick
Jun 1, 1974·The Biochemical Journal·J AirhartE A Khairallah
Jul 1, 1994·The Journal of Nutrition·T A DavisP J Reeds

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Citations

Aug 1, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·B StollD G Burrin
Mar 31, 2015·Annals of the New York Academy of Sciences·Kenneth C WelchMarshall D McCue
Jul 7, 2001·American Journal of Physiology. Endocrinology and Metabolism·H FouilletD Tomé
Mar 20, 2003·American Journal of Physiology. Endocrinology and Metabolism·Hélène FouilletDaniel Tomé
Oct 22, 2002·American Journal of Physiology. Endocrinology and Metabolism·Jill A BushTeresa A Davis
Jan 7, 2003·The Journal of Nutrition·Teresa A DavisMarta L Fiorotto
Jun 18, 2005·JPEN. Journal of Parenteral and Enteral Nutrition·Peter RittlerWolfgang H Hartl
Oct 28, 2004·American Journal of Physiology. Endocrinology and Metabolism·Cécile BosDaniel Tomé
Apr 3, 2019·American Journal of Physiology. Endocrinology and Metabolism·Robert R WolfePaul J Moughan

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