PMID: 11312685Apr 21, 2001Paper

Phenylboronic acid-salicylhydroxamic acid bioconjugates. 2. Polyvalent immobilization of protein ligands for affinity chromatography

Bioconjugate Chemistry
J P WileyM L Stolowitz

Abstract

Phenylboronic acid bioconjugates prepared from alkaline phosphatase by reaction with either 2,5-dioxopyrrolidinyl 3-[N-[3-(1,3,2-dioxaboran-2-yl)phenyl]carbamoyl]propanoate (PBA-XX-NHS) or 2,5-dioxopyrrolidinyl 6-[[3,5-di-(1,3,2-dioxaboran-2-yl)phenyl]carbonylamino]hexanoate (PDBA-X-NHS) were compared with respect to the efficiency with which they were immobilized on salicylhydroxamic acid-modified Sepharose (SHA-X-Sepharose) by boronic acid complex formation. When immobilized on moderate capacity SHA-X-Sepharose (5.4 micromol of SHA/mL of gel), PDBA-alkaline phosphatase conjugates were shown to be stable with respect to both the alkaline (pH 11.0) and acidic (pH 2.5) buffers utilized to recover anti-alkaline phosphatase during affinity chromatography. Boronic acid complex formation was compared to covalent immobilization of alkaline phosphatase on Affi-Gel 10 and Affi-Gel 15. PDBA-AP.SHA-X-Sepharose was shown to afford superior performance to both Affi-Gel 10 and Affi-Gel 15 with respect to immobilization of alkaline phosphatase, retention of anti-alkaline phosphatase and recovery of anti-alkaline phosphatase under alkaline conditions. High capacity SHA-X-Sepharose (> or = 7 micromol of SHA/mL of gel) was shown to afford super...Continue Reading

Citations

May 24, 2011·Molecular Biotechnology·Alexandra ThieleMike Schutkowski
Jul 14, 2005·Journal of Biotechnology·Russell H TaylorMary Alice Hefford
Jun 5, 2014·Journal of Medicinal Chemistry·Jonathan D AshleyBasar Bilgicer
Sep 23, 2009·Advanced Functional Materials·Julie I JayPatrick F Kiser
Apr 26, 2014·Chemistry, an Asian Journal·Matthias ArztTanja Weil
Jun 4, 2019·Chemical Society Reviews·João P M AntónioPedro M P Gois
Jan 28, 2020·Anti-cancer Agents in Medicinal Chemistry·Mitra KoraniAmirhossein Sahebkar
Sep 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Katarzyna DerwinskaClaudia Preininger
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Vladimir I Muronetz, Timo Korpela
Jun 25, 2021·Chemical Science·Saurav ChatterjeeAnupam Bandyopadhyay
Apr 7, 2017·Bioconjugate Chemistry·Arianna GennariNicola Tirelli

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