PMID: 11606193Oct 19, 2001Paper

Phenylcoumaran benzylic ether and isoflavonoid reductases are a new class of cross-reactive allergens in birch pollen, fruits and vegetables

European Journal of Biochemistry
F KaramlooS Vieths

Abstract

We investigated the biochemical function of the birch pollen allergen Bet v 6 and its role in the IgE-cross-reactivity between birch pollen and plant foods, and characterized Pyr c 5, a Bet v 6-related food allergen, from pear; the proteins were expressed as His-Tag fusion proteins in Eschershia coli and purified by Ni-chelate affinity chromatography under native conditions. Nonfusion proteins were obtained by factor Xa protease treatment. The highest degree of amino-acid sequence identity of Pyr c 5 and Bet v 6 was found with a plant protein related to a defense mechanism, which we have named phenylcoumaran benzylic ether reductase (PCBER) based on its ability to catalyze the NADPH-dependent reduction of 8-5' linked lignans such as dehydrodiconiferyl alcohol to give isodihydrodehydrodiconiferyl alcohol. Enzymatic assays with recombinant Pyr c 5 and Bet v 6 showed PCBER catalytic activity for both recombinant allergens. Both Pyr c 5 and Bet v 6 allergens had similar IgE binding characteristics in immunoblotting and enzyme allergosorbent tests (EAST), and bound IgE from 10 sera of birch-pollen-allergic patients including six pear-allergic subjects. EAST inhibition experiments with Pyr c 5 as the solid phase antigen suggested tha...Continue Reading

References

Jun 1, 1976·The Journal of Allergy and Clinical Immunology·R P Siraganian, M J Brodsky
Jun 1, 1975·Journal of Immunological Methods·R P Siraganian
Jan 1, 1992·International Archives of Allergy and Immunology·R van ReeR C Aalberse
Nov 25, 1988·Nucleic Acids Research·F Corpet
Oct 1, 1972·Immunochemistry·M Ceska, U Lundkvist
Aug 1, 1982·Allergy·N E ErikssonE Svenonius
Aug 1, 1994·International Archives of Allergy and Immunology·S ViethsA Petersen
Jan 1, 1996·In Vitro Cellular & Developmental Biology. Animal·J CapiaumontP Nabet
Dec 1, 1996·Nature Structural Biology·M GajhedeM D Spangfort
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 26, 1999·International Archives of Allergy and Immunology·K FötischS Vieths
Jul 19, 2000·International Archives of Allergy and Immunology·K Hoffmann-Sommergruber
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·F KaramlooS Vieths
Nov 1, 1997·Environmental Toxicology and Pharmacology·S Vieths

❮ Previous
Next ❯

Citations

Jul 1, 2004·Indian Journal of Clinical Biochemistry : IJCB·A B Singh, Pawan Kumar
Jul 31, 2004·Current Allergy and Asthma Reports·Nadine Mothes, Rudolf Valenta
May 5, 2012·Clinical Reviews in Allergy & Immunology·Alok Kumar VermaPremendra D Dwivedi
Feb 14, 2004·Methods : a Companion to Methods in Enzymology·Barbara Bohle, Stefan Vieths
Apr 7, 2006·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·UNKNOWN American College of Allergy, Asthma, & Immunology
Mar 3, 2004·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Riccardo AseroStefano Amato
Sep 1, 2002·Molecular Plant Pathology·Richard A DixonLiangjiang Wang
Dec 8, 2004·International Archives of Allergy and Immunology·Nadine MothesRudolf Valenta
Dec 14, 2004·International Archives of Allergy and Immunology·S T H P BolhaarL Zuidmeer
Jun 23, 2005·International Archives of Allergy and Immunology·Silvia G IrañetaVilma G Duschak
Aug 24, 2006·International Archives of Allergy and Immunology·L ZuidmeerR van Ree
Aug 3, 2005·The Journal of Clinical Investigation·Istvan BoldoghSanjiv Sur
Mar 8, 2014·Revue des maladies respiratoires·F LavaudF de Blay
Aug 30, 2002·Allergologia et immunopathologia·M SchiappoliG Passalacqua
Feb 24, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T FujimuraK Ono
May 3, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L ZuidmeerR van Ree
Jan 11, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S KaulA Hoffmann
Nov 1, 2013·Proteomics·Eva Guzmán-GarcíaSebastien Christian Carpentier
Jun 6, 2006·Allergologia et immunopathologia·Alvaro Moreno-AncilloFernando Pineda
Nov 17, 2005·Allergologia et immunopathologia·A Moreno-AncilloF Pineda
Jan 22, 2011·The Journal of Allergy and Clinical Immunology·Marija Geroldinger-SimicBarbara Bohle
Jun 12, 2016·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·P M MatricardiM Ollert
May 12, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J ZimmerS Kaul
Nov 22, 2017·Clinical Reviews in Allergy & Immunology·Akanksha SharmaPremendra D Dwivedi
Oct 28, 2004·Molecular Nutrition & Food Research·Barbara Bohle
Feb 2, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Christian Seutter von LoetzenDirk Schiller
Mar 8, 2006·Molecular Nutrition & Food Research·Jesus F CrespoStephan Scheurer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.