Phenylethanol Glycosides from Cistanche tubulosa Suppress Hepatic Stellate Cell Activation and Block the Conduction of Signaling Pathways in TGF-β1/smad as Potential Anti-Hepatic Fibrosis Agents

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Shu-Ping YouTao Liu

Abstract

Cistanche tubulosa is a traditional Chinese herbal medicine widely used for regulating immunity and phenylethanol glycosides (CPhGs) are among the primary components responsible for this activity. Previous studies have indicated the preventive and therapeutic effects of CPhGs on bovine serum albumin (BSA)-induced hepatic fibrosis in rats. The aim of the study was to evaluate the anti-hepatic fibrosis effect of CPhGs and the monomers echinacoside and acteoside by inhibiting hepatic stellate cell (HSC) activation, blocking the conduction of signaling pathways in transforming growth factor-β1 (TGF-β1)/smad, and determine their in vitro hepatoprotective activity. HSC proliferation was obviously inhibited after treatment with CPhGs (100, 50 μg/mL)/echinacoside (500, 250, 125 μg/mL)/acteoside (6, 3 μg/mL), with IC50 values of 119.125, 520.345 and 6.999 μg/mL, respectively, in the MTT assay. Different concentrations of CPhGs/echinacoside/acteoside did not affect the cellular toxicity on HSC according to lactate dehydrogenase (LDH) measurements. Different concentrations of CPhGs/echinacoside/acteoside increased the mRNA level and protein expression of smad7, and decreased the mRNA levels of smad2, smad3 and the protein expression of sm...Continue Reading

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Citations

Jan 19, 2018·Aging and Disease·Ningqun WangXianglan Jin
Dec 5, 2019·Frontiers in Chemistry·Helin XuJinli Zhang
Sep 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shi-Lei ZhangTao Liu
Aug 4, 2020·Chemical & Pharmaceutical Bulletin·Huibo LeiWeidong Zhang
May 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingjing LiuXueqin Ma
Dec 14, 2019·Biotechnology for Biofuels·Chung-Chuan HsuehBor-Yann Chen
Jun 17, 2021·Environmental Analysis, Health and Toxicology·Handule LeeKwangsik Park
Aug 3, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaowei BaoWeiquan Jin

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Methods Mentioned

BETA
PCR
nuclear translocation
Assay

Software Mentioned

iQ5 Real Time PCR Detection
SPSS
GEL DOC Image Studio

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