Phenylphosphate carboxylase: a new C-C lyase involved in anaerobic phenol metabolism in Thauera aromatica

Journal of Bacteriology
Karola Schühle, G Fuchs

Abstract

The anaerobic metabolism of phenol in the beta-proteobacterium Thauera aromatica proceeds via carboxylation to 4-hydroxybenzoate and is initiated by the ATP-dependent conversion of phenol to phenylphosphate. The subsequent para carboxylation of phenylphosphate to 4-hydroxybenzoate is catalyzed by phenylphosphate carboxylase, which was purified and studied. This enzyme consists of four proteins with molecular masses of 54, 53, 18, and 10 kDa, whose genes are located adjacent to each other in the phenol gene cluster which codes for phenol-induced proteins. Three of the subunits (54, 53, and 10 kDa) were sufficient to catalyze the exchange of 14CO2 and the carboxyl group of 4-hydroxybenzoate but not phenylphosphate carboxylation. Phenylphosphate carboxylation was restored when the 18-kDa subunit was added. The following reaction model is proposed. The 14CO2 exchange reaction catalyzed by the three subunits of the core enzyme requires the fully reversible release of CO2 from 4-hydroxybenzoate with formation of a tightly enzyme-bound phenolate intermediate. Carboxylation of phenylphosphate requires in addition the 18-kDa subunit, which is thought to form the same enzyme-bound energized phenolate intermediate from phenylphosphate wit...Continue Reading

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Citations

Oct 20, 2009·Archives of Microbiology·Sirko Schmeling, Georg Fuchs
May 25, 2013·Journal of Biotechnology·Lennart Schada von BorzyskowskiTobias J Erb
Jul 25, 2013·Journal of Biotechnology·Christiane WuenschKurt Faber
Jul 12, 2007·Dalton Transactions : an International Journal of Inorganic Chemistry·Michele Aresta, Angela Dibenedetto
Apr 21, 2009·Applied and Environmental Microbiology·Kathleen M SchleinitzGeorg Fuchs
Oct 18, 2011·Applied and Environmental Microbiology·Tobias J Erb
Sep 10, 2005·Applied and Environmental Microbiology·Romy Chakraborty, John D Coates
Sep 19, 2006·Journal of Bacteriology·Ariun NarmandakhGeorg Fuchs
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Manuel CarmonaEduardo Díaz
Oct 13, 2005·Biological Chemistry·Matthias Boll, Georg Fuchs
Oct 8, 2013·Applied and Environmental Microbiology·Tian ZhangDerek R Lovley
Apr 2, 2008·Annals of the New York Academy of Sciences·Georg Fuchs
Apr 17, 2008·Environmental Microbiology·Umakanth KunapuliRainer U Meckenstock
Mar 16, 2012·FEMS Microbiology Ecology·Claudia KellermannChristian Griebler
Feb 17, 2019·Journal of Computational Chemistry·Oscar A Douglas-GallardoEsteban Vöhringer-Martinez
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Jan 13, 2021·Environmental Science & Technology·Siyuan ZhaiSpyros G Pavlostathis
Feb 26, 2021·Applied Microbiology and Biotechnology·M Concetta TomeiLorenzo Brusetti

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