Pheophorbide Derivatives Isolated from Açaí Berries (Euterpea oleracea ) Activate an Antioxidant Response Element In Vitro

Natural Product Communications
Suneel K KandagatlaGregory M Raner

Abstract

Activity-guided fractionation was used to isolate and identify two components of the Brazilian açaí berry (Euterpe oleracea Mart.) with the ability to induce antioxidant response element (ARE)-dependent gene transcription in human hepatoma (HepG2) cells. Using an ARE-Luciferase reporter construct in cultured HepG2 cells, a suite of fractions from dried and powdered açaí berries were evaluated for transcriptional up-regulation of the luciferase gene. Active fractions were further refined until several pure compounds were isolated and identified. These compounds belong to the pheophorbide class of molecules, and are composed of the methyl and ethyl esters of the parent pheophorbide A, all of which are classified as photosensitizers. Using standard pheophorbides, dose response studies were carried out, and ARE-activation could be observed at concentrations as low as 8.2 μM and 16.9 μM for pheophorbide A methyl ester and pheophorbide A, respectively. These studies not only suggest a possible source of antioxidant properties for the açaí berry, but may also explain the recently identified photosensitizing abilities of açaí products as well.

References

Feb 21, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Shin-Pei YangGregory M Raner
Oct 26, 2006·Journal of Agricultural and Food Chemistry·Alexander G SchaussJames P Kababick
Dec 31, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Ngoc NgoMary F Paine
Apr 9, 2010·The Journal of Nutrition·Paul E MilburyJeffrey B Blumberg
Dec 1, 2011·Journal of Clinical Biochemistry and Nutrition·Joyce Ferreira da Costa GuerraMaria Lúcia Pedrosa
Dec 6, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Roberto Soares de MouraSamuel Santos Valenca
Aug 14, 2012·Pharmacology & Therapeutics·Christina S WonMary F Paine
Dec 27, 2016·Journal of Photochemistry and Photobiology. B, Biology·Victoria Monge-FuentesRicardo Bentes Azevedo
Apr 4, 2018·BMC Complementary and Alternative Medicine·Jéssica Alessandra-PeriniDaniel Escorsim Machado

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