Pheromone Binding Protein EhipPBP1 Is Highly Enriched in the Male Antennae of the Seabuckthorn Carpenterworm and Is Binding to Sex Pheromone Components

Frontiers in Physiology
Ping HuJing Tao

Abstract

The seabuckthorn carpenterworm moth Eogystia hippophaecolus is a major threat to seabuckthorn plantations, causing considerable ecological and economic losses in China. Transcriptomic analysis of E. hippophaecolus previously identified 137 olfactory proteins, including three pheromone-binding proteins (PBPs). We investigated the function of E. hippophaecolus PBP1 by studying its mRNA and protein expression profiles and its binding ability with different compounds. The highest levels of expression were in the antennae, particularly in males, with much lower levels of expression in the legs and external genitals. Recombinant PBP1 showed strong binding to sex-pheromone components, suggesting that antennal EhipPBP1 is involved in binding sex-pheromone components during pheromone communication.

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Datasets Mentioned

BETA
KX655931

Methods Mentioned

BETA
PCR
Fluorescence
electrophoresis
enzyme-linked immunosorbent assay
fluorescence binding
NMR

Software Mentioned

Blast
SWISS MODEL
SignalP
Rad CFX Manager
SWISS
Open Reading Frame Finder
ProMod3
MODEL
SPSS
HHBlits

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