Pheromone biosynthetic pathways: PBAN-regulated rate-limiting steps and differential expression of desaturase genes in moth species

Insect Biochemistry and Molecular Biology
Oren TsfadiaAda Rafaeli

Abstract

We combine the use of labeled precursors with enzyme inhibitors to decipher the biosynthetic pathway of pheromone biosynthesis and the rate-limiting step/s that are regulated by pheromone biosynthesis activating neuropeptide (PBAN). We demonstrate that Plodia interpunctella is able to utilize hexadecanoic acid, and to a lesser extent tetradecanoic acid, for the biosynthesis of the main pheromone component (Z,E)-9,12-tetradecadienyl acetate. This indicated that the main pathway involves a Delta11 desaturase, chain shortening, followed by a Delta12 desaturase, but that a functional Delta9 desaturase could also be utilized. Using reverse transcription-quantitative real-time polymerase chain reaction (RT-QPCR) we distinguish two out of nine possible desaturase gene transcripts in P. interpunctella that are expressed at the highest levels. The rate-limiting step for PBAN-stimulation was studied in two moth species so as to compare the biosynthesis of a diene (P. interpunctella) and a monoene (Helicoverpa armigera) main pheromone component. In both species, incorporation of label from the (13)C sodium acetate precursor was activated by PBAN whereas no stimulatory action was observed in the incorporation of the precursors: (13)C malon...Continue Reading

References

Jan 1, 1990·Archives of Insect Biochemistry and Physiology·G ArsequellF Camps
Jan 15, 1994·European Journal of Biochemistry·J HaK H Kim
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·D C KnippleW L Roelofs
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·R DalleracC Wicker-Thomas
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Weitian LiuWendell L Roelofs
Sep 6, 2002·Insect Biochemistry and Molecular Biology·G HaoW l Roelofs
Sep 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Wendell L RoelofsCharles E Linn
Jan 18, 2003·Insect Biochemistry and Molecular Biology·Man-Yeon ChoiRussell A Jurenka
Jan 18, 2003·Insect Biochemistry and Molecular Biology·Weitian LiuWendell L Roelofs
Jan 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Russell A JurenkaGemma Fabrias
Mar 29, 2003·The Journal of Organic Chemistry·Gemma VillorbinaGemma Fabriàs
Jul 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wendell L Roelofs, Alejandro P Rooney
Jul 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Man-Yeon ChoiRussell Jurenka
Jun 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ken'ichi MotoShogo Matsumoto
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J D TangW L Roelofs
Aug 1, 2006·Insect Biochemistry and Molecular Biology·Montserrat SerraGemma Fabriàs
Oct 10, 2006·Insect Biochemistry and Molecular Biology·Montserrat SerraPeter H Buist
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Bingye XueWendell L Roelofs

❮ Previous
Next ❯

Citations

Sep 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Rachel Bober, Ada Rafaeli
Dec 27, 2008·Frontiers in Zoology·Astrid T GrootDavid G Heckel
Feb 9, 2010·Bioscience, Biotechnology, and Biochemistry·Shogo Matsumoto
Feb 18, 2014·Journal of Chemical Ecology·Minmin SongClaus Tittiger
Sep 18, 2012·Insect Biochemistry and Molecular Biology·Stephen P Foster, Karin G Anderson
Oct 6, 2010·Biochimica Et Biophysica Acta·Vincent RiouxPhilippe Legrand
May 23, 2008·General and Comparative Endocrinology·Ada Rafaeli
Nov 14, 2015·Annual Review of Entomology·Astrid T GrootDavid G Heckel
Aug 18, 2010·Communicative & Integrative Biology·J Joe HullShogo Matsumoto
Jul 26, 2017·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Michal TupecIva Pichová

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.