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phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America

Oct 1, 1975

H SchusterB Geschke

PMID: 670

Abstract

Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside...read more

Mentioned in this Paper

Hydrogen-Ion Concentration
Strepto-Hefa
Magnesium
Soluble
DNA Biosynthetic Process
Purification Aspects
Bacteriophage phi X 174
Entamoeba coli
Rifampin
In Vitro [Publication Type]
Paper Details
References
  • References12
  • Citations18
12
  • References12
  • Citations18
12

phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America

Oct 1, 1975

H SchusterB Geschke

PMID: 670

DOI:

Abstract

Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside...read more

Mentioned in this Paper

Hydrogen-Ion Concentration
Strepto-Hefa
Magnesium
Soluble
DNA Biosynthetic Process
Purification Aspects
Bacteriophage phi X 174
Entamoeba coli
Rifampin
In Vitro [Publication Type]

Related Papers

Paper Details
References
  • References12
  • Citations18
12
  • References12
  • Citations18
12
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