PMID: 670Oct 1, 1975

phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
H SchusterB Geschke

Abstract

Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside triphosphates, and ATP, and is stimulated by KCl. Such soluble enzyme fractions were prepared from E. coli strains carrying the prophage mutant P1bac, in which the viral dnaB analog (ban) protein is expressed constitutively, or P1bacban, in which the expression of ban protein is prevented. DNA-synthesizing activity of ban protein containing fractions from wild-type or dnaB(P1bac) lysogens was more temperature-resistant than that from E. coli containing only wild-type dnaB protein, whereas that from dnaB(P1bacban) lysogens of dnaB cells was extremely thermolabile. It is suggested that the temperature-resistant DNA synthesis with fractions from P1bac lysogens is mediated by the P1 ban protein.

References

May 25, 1975·Journal of Molecular Biology·R D'AriM B Yarmolinsky
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M WrightJ Hurwitz
Jan 1, 1972·Molecular & General Genetics : MGG·D Beyersmann, H Schuster
Apr 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·W WicknerA Kornberg
Aug 6, 1973·Biochemical and Biophysical Research Communications·M Koiyama, E Milewski
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S Wickner, J Hurwitz
Dec 13, 1974·Science·R SchekmanA Kornberg
Nov 14, 1968·Journal of Molecular Biology·R A Steinberg, D T Denhardt
Jan 1, 1970·Molecular & General Genetics : MGG·E Lanka, H Schuster
Jan 1, 1971·Molecular & General Genetics : MGG·M Mikolajczyk, H Schuster
Jan 1, 1971·Molecular & General Genetics : MGG·J A Wechsler, J D Gross

Citations

Jun 15, 1976·Molecular & General Genetics : MGG·W L Staudenbauer
Feb 28, 1977·Molecular & General Genetics : MGG·H SchusterC Edelbluth
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E LankaH Schuster
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E LankaH Schuster
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E ScherzingerM Bagdasarian
Feb 11, 1982·Nucleic Acids Research·G Hillenbrand, W L Staudenbauer
Jun 11, 1994·Nucleic Acids Research·R Díaz OrejasE Lanka
Oct 24, 1981·Nucleic Acids Research·T W BöldickeW L Staudenbauer
Feb 1, 1987·Mutation Research·V Murray
Jan 5, 1989·Journal of Molecular Biology·T HeinzelH Schuster

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