Phleomycin resistance as a dominant selectable marker for plant cell transformation

Plant Molecular Biology
P PerezJ Perret

Abstract

Tobacco cells are sensitive to bleomycin and phleomycin. The Tn5 and the Streptoalloteichus hindustanus (Sh) bleomycin resistance ('Ble') genes conferring resistance to these antibiotics have each been inserted into two plant expression vectors. They are flanked by the nopaline synthase (nos) or the cauliflower mosaic virus (CaMV) 35S promoters on one side, and by the nos polyadenylation signal on the other. These four chimaeric genes were introduced into the binary transformation vector pGA 492, which were thereafter mobilized into Agrobacterium tumefaciens strain LBA 4404. The resulting strains were used to transform Nicotiana tabacum cv. Xanthi nc using the leaf disc transformation procedure. In all cases, phleomycin- and bleomycin-resistant tobacco plants were regenerated from transformed cells under selective conditions; however, the highest frequency of rooted plants was obtained when transformation was carried out with the 'Sh Ble' gene under the control of the 35S promoter. Phleomycin resistance was stably transmitted to sexual offspring as a dominant nuclear trait as confirmed by Southern blotting.

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Citations

Sep 1, 1995·Plant Molecular Biology·R V SontiE R Signer
Jan 23, 2004·Journal of Biotechnology·Brian Miki, Sylvia McHugh
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·R V SontiE R Signer
Mar 25, 2011·Cellular and Molecular Life Sciences : CMLS·Rosina Giordano-Santini, Denis Dupuy
Jan 16, 2007·FEBS Letters·Laurent CambordeIsabelle Jupin
Sep 16, 2008·Journal of Plant Physiology·Isaac Kirubakaran Sundar, Natarajan Sakthivel
Jun 26, 2007·Protein Expression and Purification·Masaki YamaguchiTadayoshi Taniyama
Apr 21, 2016·Plant Science : an International Journal of Experimental Plant Biology·Alessandra RogatoMaurizio Chiurazzi
Oct 27, 2007·Environmental Biosafety Research·Alessandra PontiroliJean-Michel Monier

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