PhlG mediates the conversion of DAPG to MAPG in Pseudomonas fluorescens 2P24.

Scientific Reports
Ming-Min ZhaoHong-You Zhou

Abstract

The antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG), produced by the Gram-negative rod-shaped bacterium Pseudomonas fluorescens 2P24, is active against various soil-borne bacterial and fungal pathogens that cause plant diseases. Biosynthesis of 2,4-DAPG is controlled by regulating expression of the phlACBD operon at the post-transcriptional level. The phlG gene is located between the phlF and phlH genes, upstream of the phlACBD biosynthetic operon. Herein, we cloned the phlG gene, generated a phlG deletion mutant, and investigated its regulatory role in 2,4-DAPG biosynthesis. The results showed that deletion of phlG had no effect on the biosynthesis of 2,4-DAPG, but it affected conversion of 2,4-DAPG to its precursor monoacetylphloroglucinol (MAPG). The global regulatory factor encoded by gacS positively regulated expression of phlG, while rsmE negatively regulated its expression. Deleting phlG did not alter the ability of the bacterium to colonise plants or promote plant growth. These results suggest that phlG collaborates with other factors to regulate production of the antibiotic 2,4-DAPG in P. fluorescens 2P24.

References

Feb 1, 1972·Genetical Research·V A Stanisich, B W Holloway
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·A SarniguetJ E Loper
Mar 11, 2005·Nature Reviews. Microbiology·Dieter Haas, Geneviève Défago
Mar 24, 2005·Molecular Plant-microbe Interactions : MPMI·Maria Péchy-TarrChristoph Keel
Jun 28, 2005·Nature Biotechnology·Ian T PaulsenJoyce E Loper
Nov 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth KayDieter Haas
Jul 14, 2012·Bioscience, Biotechnology, and Biochemistry·Hiroki SaitouKiyofumi Maruyama

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Datasets Mentioned

BETA
DQ083928.1

Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad Prism

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