Phloretin, an Apple Polyphenol, Inhibits Pathogen-Induced Mucin Overproduction.

Molecular Nutrition & Food Research
Rahel L BirruGeorge D Leikauf

Abstract

Bacterial infection induces mucus overproduction, contributing to acute exacerbations and lung function decline in chronic respiratory diseases. A diet enriched in apples may provide protection from pulmonary disease development and progression. This study examined whether phloretin, an apple polyphenol, inhibits mucus synthesis and secretion induced by the predominant bacteria associated with chronic respiratory diseases. The expression of mucus constituent mucin 5AC (MUC5AC) in FVB/NJ mice and NCI-H292 epithelial cells is analyzed. Nontypeable Haemophilus influenzae (NTHi)-infected mice developed increased MUC5AC mRNA, which a diet containing phloretin inhibited. In NCI-H292 cells, NTHi, Moraxella catarrhalis, Streptococcus pneumoniae, and Pseudomonas aeruginosa increased MUC5AC mRNA, which phloretin inhibited. Phloretin also diminished NTHi-induced MUC5AC protein secretion. NTHi-induced increased MUC5AC required toll-like receptor 4 (TLR4) and NADH oxidase 4 (NOX4) signaling and subsequent activation of the epidermal growth factor receptor (EGFR)/mitogen-activated protein kinase (MAPK) pathway. Phloretin inhibited NTHi-induced TLR4/NOX4 and EGFR/MAPK signaling, thereby preventing increased MUC5AC mRNA. EGFR activation can al...Continue Reading

References

Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K TakeyamaJ A Nadel
Jan 5, 2002·Biochimica Et Biophysica Acta·E B YangP Mack
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaorong WangJames M Wilson
Jul 26, 2002·Critical Reviews in Biochemistry and Molecular Biology·Matthew F LeemanGraeme I Murray
Oct 16, 2003·Journal of Agricultural and Food Chemistry·Ki Won LeeChang Yong Lee
Jan 27, 2004·Infection and Immunity·Judith BrangerTom van der Poll
May 1, 2004·American Journal of Respiratory and Critical Care Medicine·Lesley M ButlerStephanie J London
May 4, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Matt X G ShaoJay A Nadel
Nov 9, 2004·American Journal of Respiratory and Critical Care Medicine·Hitesh S DeshmukhGeorge D Leikauf
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catharina W WielandTom van der Poll
May 3, 2007·The Laryngoscope·Mahmoud S Ali, Jeffrey P Pearson
Nov 17, 2007·American Journal of Respiratory Cell and Molecular Biology·Hitesh S DeshmukhGeorge D Leikauf
Nov 27, 2007·Biochemical and Biophysical Research Communications·Huahao ShenJian-Dong Li
Jun 10, 2008·American Journal of Respiratory Cell and Molecular Biology·Hyun Jik KimJoo-Heon Yoon
Jul 12, 2008·American Journal of Respiratory Cell and Molecular Biology·Becky A MercerJeanine M D'Armiento
Oct 2, 2008·The European Respiratory Journal·P-R Burgel, J A Nadel
Nov 29, 2008·The New England Journal of Medicine·Sanjay Sethi, Timothy F Murphy
Jan 31, 2009·American Journal of Respiratory and Critical Care Medicine·Gordon J GaschlerMartin R Stämpfli
Aug 8, 2009·American Journal of Respiratory and Critical Care Medicine·Hitesh S DeshmukhGeorge D Leikauf
Jan 16, 2010·American Journal of Respiratory and Critical Care Medicine·John V GosselinkJames C Hogg
Jun 8, 2010·The European Respiratory Journal·C A HewsonS L Johnston
Apr 27, 2011·The Journal of Clinical Investigation·Paul ElkingtonJon S Friedland
Nov 2, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M WoodheadUNKNOWN Joint Taskforce of the European Respiratory Society and European Society for Clinical Microbiology and Infectious Diseases
Nov 2, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M WoodheadUNKNOWN Joint Taskforce of the European Respiratory Society and European Society for Clinical Microbiology and Infectious Diseases
Oct 19, 2011·Journal of Cellular Biochemistry·Stéphane BarthelemiSandrine Lorimier
Oct 8, 2013·Antioxidants & Redox Signaling·Karen BernardVictor J Thannickal
Dec 7, 2013·Experimental & Molecular Medicine·Beom Seok Park, Jie-Oh Lee
Jan 2, 2016·Redox Biology·David E Heppner, Albert van der Vliet
Jun 18, 2016·International Journal of Chronic Obstructive Pulmonary Disease·Hyeon-Kyoung KooWoo Jin Kim
Sep 17, 2016·F1000Research·Bhuminder SinghRobert J Coffey
Nov 20, 2016·Cold Spring Harbor Perspectives in Biology·Ximena M Bustamante-Marin, Lawrence E Ostrowski
Mar 1, 2017·Frontiers in Immunology·Wen-Chung HuangChian-Jiun Liou
May 11, 2017·International Journal of Inflammation·Anuhya Sharma KonduruJian-Dong Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.