Phloretin suppresses thrombin-mediated leukocyte-platelet-endothelial interactions

Molecular Nutrition & Food Research
Min Soo KimYoung-Hee Kang

Abstract

Thrombin playing a pivotal role in coagulation cascade may influence the onset and progression of atherosclerosis as a pro-inflammatory mediator. This study investigated whether phloretin found in apple tree leaves, severed a linkage between thrombosis and atherosclerosis by thrombin. Human endothelial cells were pre-treated with 1-20 μM phloretin and stimulated with 10 U/mL thrombin. Phloretin attenuated adhesion of THP-1 monocytes and platelets to thrombin-inflamed endothelial cells with concurrent inhibition of protease-activated receptor (PAR-1) induction. The thrombin induction of endothelial CD40, endothelial integrin β3 and P-selectin, and monocytic CD40L was dampened by phloretin. Additionally, phloretin inhibited monocyte secretion of MCP-1, IL-6 and IL-8 responsible for pro-inflammatory activity of thrombin inducing endothelial CD40. The monocyte COX-2 induction and PGE2 secretion due to thrombin were down-regulated by phloretin, deterring endothelial CD40 expression. Thrombin promoted production of PAI-1 and tissue factor in monocytes was attenuated by phloretin through blocking PAR-1 and CD40. Thrombin up-regulated the induction of endothelial connective tissue growth factor independent of PAR-1 activation, which wa...Continue Reading

References

Jan 7, 2000·The American Journal of Pathology·U SchönbeckP Libby
Apr 3, 2001·The Journal of Biological Chemistry·G SoslauZ M Ruggeri
Apr 12, 2003·Critical Care Medicine·Steven Idell
Sep 13, 2003·Chest·Lawrence F Brass
Mar 12, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Iwona CichaChristoph D Garlichs
Jul 23, 2005·Archives of Biochemistry and Biophysics·Arthur J Chu
Aug 22, 2006·Current Molecular Medicine·J F Viles-GonzalezJ J Badimon
Sep 6, 2006·Circulation·Andrew J LegerAthan Kuliopulos
May 14, 2008·Current Medicinal Chemistry·Esther ZorioAmparo Estellés
Aug 30, 2008·The New England Journal of Medicine·Bruce Furie, Barbara C Furie
Feb 21, 2009·Cardiovascular Research·Julian Ilcheff BorissoffHugo ten Cate
Jun 23, 2009·Journal of Cellular and Molecular Medicine·Harald F Langer, Triantafyllos Chavakis
Jan 13, 2010·Nature Reviews. Cardiology·Kevin J Woollard, Frederic Geissmann
Aug 3, 2010·The International Journal of Biochemistry & Cell Biology·Matthew D Linden, Denise E Jackson
Aug 11, 2010·Circulation·Julian Ilcheff BorissoffHenri M H Spronk
Sep 30, 2010·Recent Patents on Cardiovascular Drug Discovery·Jing YangDietmar Seiffert
Apr 27, 2011·Expert Review of Cardiovascular Therapy·Giovanni CimminoPaolo Golino
May 21, 2011·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Elena M EgorinaJohn-Bjarne Hansen
Nov 15, 2011·Critical Care Medicine·Abderrezak BouchamaMohammed Dehbi
Feb 14, 2012·Pharmacology & Therapeutics·Hannah Lee, Justin R Hamilton
Apr 12, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Sara Martínez de LizarrondoJosune Orbe

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Citations

Jan 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bu Young Choi

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