Phlorofucofuroeckol B suppresses inflammatory responses by down-regulating nuclear factor κB activation via Akt, ERK, and JNK in LPS-stimulated microglial cells

International Immunopharmacology
Dong-Kyung YuHyeung-Rak Kim

Abstract

Microglial activation has been implicated in many neurological disorders for its inflammatory and neurotrophic effects. In this study, we investigated the effects of phlorofucofuroeckol B (PFF-B) isolated from Ecklonia stolonifera, on the production of inflammatory mediators in lipopolysaccharide (LPS)-stimulated microglia. PFF-B decreased secretion of pro-inflammatory cytokines including tumor necrosis factor α, interleukin (IL)-1β, and IL-6 and the expression of pro-inflammatory proteins such as cyclooxygenase-2 and inducible nitric oxide synthase in LPS-stimulated BV-2 cells. Profoundly, PFF-B inhibited activation of nuclear factor kappaB (NF-κB) by preventing the degradation of inhibitor κB-α (IκB-α), which led to prevent the nuclear translocation of p65 NF-κB subunit. Moreover, PFF-B inhibited the phosphorylation of Akt, ERK, and JNK. These results indicate that the anti-inflammatory effect of PFF-B on LPS-stimulated microglial cells is mainly regulated by the inhibition of IκB-α/NF-κB and Akt/ERK/JNK pathways. Our study suggests that PFF-B can be considered as a therapeutic agent against neuroinflammation by inhibiting microglial activation.

References

Jan 1, 1994·Developmental Neuroscience·D GiulianP A Rutecki
Aug 1, 1996·Trends in Neurosciences·G W Kreutzberg
Jan 1, 1996·Advances in Neuroimmunology·J Zielasek, H P Hartung
Aug 5, 2000·Free Radical Biology & Medicine·Y M Janssen-HeiningerP A Baeuerle
Mar 21, 2001·Cellular Signalling·M Guha, N Mackman
May 17, 2007·Current Medicinal Chemistry·S Thameem DheenEng-Ang Ling
Dec 14, 2007·Annals of the New York Academy of Sciences·Stephen D Skaper
Aug 13, 2008·Immunology Letters·Eugene Y Kim, Kamal D Moudgil
Apr 3, 2009·Journal of Agricultural and Food Chemistry·A-Reum KimHyeung-Rak Kim
Oct 14, 2009·Frontiers in Neuroendocrinology·Joshua P ThalerBrent E Wisse
Jun 25, 2010·Endocrinology·Joshua P Thaler, Michael W Schwartz
Dec 29, 2011·The Journal of Clinical Investigation·Joshua P ThalerMichael W Schwartz
Oct 4, 2012·BMC Complementary and Alternative Medicine·Eun-Ji JoungHyeung-Rak Kim

❮ Previous
Next ❯

Citations

Apr 25, 2018·Biomedical Engineering Online·Chong NingShi-Ying Huang
Mar 16, 2017·Natural Product Reports·John W BluntMichèle R Prinsep
Jun 27, 2019·International Journal of Molecular Sciences·Maria Cristina BarbalaceCristina Angeloni
Sep 21, 2018·Journal of Neuroinflammation·Hye Yeon NamHyang-Sook Hoe
Jun 21, 2020·International Journal of Molecular Sciences·Jihyun ParkKwan-Kyu Park
Nov 22, 2020·Marine Drugs·Lei CaoHyeung-Rak Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Alzheimer's Disease: Microglia

Microglia are a type of glial cell found throughout the brain and spinal cord. Microglia have been found to be associated with Alzheimer's disease development and progression. Here are the latest discoveries pertaining to Alzheimer's disease and microglia.

Alzheimer's Disease: Transcription

Impaired transcription is associated with the pathogenesis and progression of conditions such as Alzheimer's disease (AD). Here are the latest discoveries pertaining to transcription and AD.