Phloroglucinol functions as an intracellular and intercellular chemical messenger influencing gene expression in Pseudomonas protegens

Environmental Microbiology
Jennifer C CliffordJoyce E Loper

Abstract

Bacteria can be both highly communicative and highly competitive in natural habitats and antibiotics are thought to play a role in both of these processes. The soil bacterium Pseudomonas protegens Pf-5 produces a spectrum of antibiotics, two of which, pyoluteorin and 2,4-diacetylphloroglucinol (DAPG), function in intracellular and intercellular communication, both as autoinducers of their own production. Here, we demonstrate that phloroglucinol, an intermediate in DAPG biosynthesis, can serve as an intercellular signal influencing the expression of pyoluteorin biosynthesis genes, the production of pyoluteorin, and inhibition of Pythium ultimum, a phytopathogenic oomycete sensitive to pyoluteorin. Through analysis of RNAseq data sets, we show that phloroglucinol had broad effects on the transcriptome of Pf-5, significantly altering the transcription of more than two hundred genes. The effects of nanomolar versus micromolar concentrations of phloroglucinol differed both quantitatively and qualitatively, influencing the expression of distinct sets of genes or having opposite effects on transcript abundance of certain genes. Therefore, our results support the concept of hormesis, a phenomenon associated with signalling molecules th...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·A SarniguetJ E Loper
Mar 27, 1999·Journal of Bacteriology·B Nowak-ThompsonJ E Loper
Sep 11, 1999·Journal of Bacteriology·A R Horswill, J C Escalante-Semerena
Jun 28, 2002·Cell·Bonnie L Bassler
Dec 11, 2002·European Journal of Biochemistry·Matthias BrockWolfgang Buckel
Mar 6, 2003·Neuroscience Letters·Christian RamakersAntoon F M Moorman
Mar 1, 1962·Journal of Bacteriology·R W HANSEN, J A HAYASHI
Mar 12, 2004·Annual Review of Phytopathology·L S PiersonE A Pierson
Apr 7, 2004·Applied and Environmental Microbiology·Monika MaurhoferChristoph Keel
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paula J WildermanMichael L Vasil
Mar 11, 2005·Nature Reviews. Microbiology·Dieter Haas, Geneviève Défago
Apr 14, 2005·Journal of the American Chemical Society·Jihane AchkarJ W Frost
Jun 28, 2005·Nature Biotechnology·Ian T PaulsenJoyce E Loper
Sep 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Pieter C DorresteinChristopher T Walsh
Feb 25, 2006·Science·Andrew Camilli, Bonnie L Bassler
Sep 1, 2006·Current Opinion in Microbiology·Julian DaviesGrace Yim
Mar 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Grace YimJulian Davies
Mar 18, 2008·Applied and Environmental Microbiology·Joyce E LoperHarald Gross
Feb 25, 2009·Nucleic Acids Research·J M RuijterA F M Moorman
Oct 22, 2009·Natural Product Reports·Harald Gross, Joyce E Loper
Jul 27, 2011·Chemical Reviews·Diego RomeroRoberto Kolter
Nov 22, 2011·Applied Microbiology and Biotechnology·Fang Yang, Yujin Cao
Dec 24, 2011·Nucleic Acids Research·Victor M MarkowitzNikos C Kyrpides
Mar 7, 2012·Microbiology and Molecular Biology Reviews : MMBR·Pol Nadal JimenezWim J Quax

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Citations

Sep 10, 2016·Frontiers in Plant Science·Jordan VacheronClaire Prigent-Combaret
Jul 23, 2019·Genetic Epidemiology·Siyun LiuTao Yu
Jun 21, 2019·Antibiotics·Thomas C McLeanRebecca Devine
May 5, 2021·Journal of Industrial Microbiology & Biotechnology·Colin Charles Barber, Wenjun Zhang

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