PMID: 7545243Oct 1, 1995Paper

Phorbol ester-induced down modulation of tailless CD4 receptors requires prior binding of gp120 and suggests a role for accessory molecules

Journal of Virology
H GoldingC Lapham

Abstract

The entry of human immunodeficiency virus type 1 into cells proceeds via a fusion mechanism that is initiated by binding of the viral glycoprotein gp120-gp41 to its cellular receptor CD4. Species- and tissue-specific restrictions to viral entry suggested the participation of additional membrane components in the postbinding fusion events. In a previous study (H. Golding, J. Manischewitz, L. Vujcic, R. Blumenthal, and D. Dimitrov, J. Virol. 68:1962-1968, 1994), it was found that phorbol myristate acetate (PMA) inhibits human immunodeficiency virus type 1 envelope-mediated cell fusion by inducing down modulation of an accessory component(s) in the CD4-expressing cells. The fusion inhibition was seen in a variety of cells, including T-cell transfectants expressing engineered CD4 receptors (CD4.401 and CD4.CD8) which are not susceptible to down modulation by PMA treatment. In the current study, it was found that preincubation of A2.01.CD4.401 cells with soluble monomeric gp120 for 1 h at 37 degrees C primed them for PMA-induced down modulation (up to 70%) of the tailless CD4 receptors. The gp120-priming effect was temperature dependent, and the down modulation may have occurred via clathrin-coated pits. Importantly, nonhuman cell l...Continue Reading

References

Aug 1, 1991·The Journal of Experimental Medicine·Q J Sattentau, J P Moore
Mar 1, 1991·The Journal of Experimental Medicine·A Pelchen-MatthewsM Marsh
Feb 1, 1989·The Journal of Cell Biology·J E Heuser, R G Anderson
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Citations

Mar 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anthony D CristilloBarbara E Bierer
Mar 18, 2003·AIDS Research and Human Retroviruses·Dennis SheeterJacques Corbeil
Feb 11, 2015·BioMed Research International·Huan LiuShibo Jiang
Jun 15, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rui André Saraiva RaposoWilliam James
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·X XiaoD S Dimitrov
May 19, 2000·Experimental and Molecular Pathology·X XiaoD S Dimitrov

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