Phorbol ester-induced translocation of PKC epsilon to the nucleus in fibroblasts: identification of nuclear PKC epsilon-associating proteins

FEBS Letters
Tian-Rui Xu, M G Rumsby

Abstract

We show that phorbol ester treatment of NIH 3T3 fibroblasts induces rapid translocation of PKC from a perinuclear site to the nucleus, extending findings in PC12 and NG108-15 cells and in myocytes. We have immunoprecipitated the PKC from nuclei isolated from phorbol ester-treated fibroblasts and identified six proteins which associate with nuclear PKC. These have been characterised as matrin 3, transferrin, Rac GTPase activating protein 1, vimentin, beta-actin and annexin II by MALDI-TOF-MS. We have confirmed that these proteins associate with PKC by gel overlay and/or dot blotting assays. The role of these PKC-associating proteins in the nucleus and their interaction with PKC are considered.

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Citations

Mar 29, 2011·Pharmacological Reports : PR·Ewa TotońMaria Rybczyńska
Jul 4, 2006·Biochimica Et Biophysica Acta·Yasuhide HibinoKoichi Hiraga
Oct 5, 2010·Biochimica Et Biophysica Acta·Carlos EnrichThomas Grewal
Jan 18, 2011·Biochimica Et Biophysica Acta·Suman DasguptaSamir Bhattacharya
Nov 15, 2012·Radiation Research·Katrina M WatersThomas J Weber
May 20, 2008·Biochimica Et Biophysica Acta·Nasim WarwarRafael Nesher
Jun 21, 2006·The Journal of Biological Chemistry·Viktoria KheifetsDaria Mochly-Rosen
Feb 17, 2018·Biochemical Pharmacology·Zhongwei Liu, Raouf A Khalil
Apr 1, 2006·Biochimica Et Biophysica Acta·Alberto M MartelliFrancesco Antonio Manzoli

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