PMID: 8972047Oct 1, 1996Paper

Phorbol ester stimulation of equine macrophage cultures alters expression of equine infectious anemia virus

Veterinary Microbiology
D C SellonFrederick J Fuller

Abstract

Equine infectious anemia virus (EIAV) is a lentivirus that replicates predominantly in mature tissue macrophages. Viral expression is strongly influenced by the state of differentiation of the host cell. While blood monocytes can be infected, viral transcription is limited until the cell differentiates into a mature macrophage. Activation of mature macrophages infected with EIAV might also alter viral expression, presumably through binding of cellular transcription factors to viral nucleic acid sequences within the long terminal repeat (LTR). Using DNA amplification techniques, we compared LTR sequences of U.S. field strains of EIAV to sequences of a laboratory adapted strain of the virus. All field strain sequences were more closely related to Wyoming strain than to the Malmquist laboratory adapted strain or a previously sequenced infectious molecular clone of EIAV. Primary equine monocyte-derived macrophage cultures were infected with virulent and avirulent strains of EIAV and the effects of macrophage stimulation on EIAV expression were determined. Stimulation of macrophages with phorbol ester activated the cells to secrete tumor necrosis factor alpha (TNF alpha). This activation signal also resulted in a significant downreg...Continue Reading

References

Feb 1, 1994·The Journal of General Virology·S L PayneF Fuller
Aug 1, 1993·The Veterinary Clinics of North America. Equine Practice·D C Sellon

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Citations

Apr 21, 2001·BMC Blood Disorders·Rafael NunezKarsten Feige
Jun 17, 1998·Journal of Biomedical Science·W Maury

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