PMID: 6967217May 1, 1980Paper

Phorbol esters induce differentiation in human malignant T lymphoblasts

Proceedings of the National Academy of Sciences of the United States of America
K Nagasawa, T W Mak

Abstract

At nanomolar concentrations, phorbol ester, a class of potent tumor promoters, can promote differentiation in the human malignant T-lymphoblastic cell line MOLT-3. The optimal dose for induction, as measured by the increase of the number of cells containing sheep erythrocyte receptors (E-rosette assay), is between 8 and 16 nM 12-O-tetradecanoylphorbol 13-acetate (TPA), although there were significant increases of E-rosette-positive (E(+)) cells at concentrations as low as 1.6 nM TPA. The induction was linear for 4 days, then it reached a plateau. This induction was independent of the cell densities of the cultures, and the viability of the E(+) cells remained high (95-100%) even after 10 days of culture in the presence of the tumor promoters. The E(+) cells, when measured with the more stable 2-aminoethylisothiouronium bromide E-rosette assay, indicated that virtually all (75-95%) of the MOLT-3 cells became E(+) by 4 days in culture. This induction by TPA was also accompanied by a dramatic drop in the plating efficiencies and a reduction in DNA synthesis. Examination of phorbol and other phrobol esters indicated that the ability to induce these cells correlated well with the tumor-promoting activities of these compounds, becaus...Continue Reading

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Citations

Jun 1, 1992·Calcified Tissue International·T Ringbom-Anderson, K E Akerman
May 1, 1984·Journal of Clinical Immunology·J OkamuraE W Gelfand
Oct 15, 1982·Biochemical and Biophysical Research Communications·P PlevaniE Ginelli
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Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T Shibuya, T W Mak
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