PMID: 8604000Feb 1, 1996Paper

Phorbol myristate acetate-induced NADPH oxidase activity in human neutrophils: only half the story has been told

Journal of Leukocyte Biology
H LundqvistClaes Dahlgren

Abstract

The paradigm for activation of the neutrophil NADPH oxidase with the protein kinase C activator phorbol myristate acetate (PMA) states that the oxidase assembles the plasma membrane and that the metabolites generated are released extracellularly. This paradigm is challenged by the results presented. Most of the PMA-induced oxidase activity measured as chemiluminescence and dichlorofluorescein fluorescence was insensitive to scavengers of superoxide anion and hydrogen peroxide. This indicates that oxidase activity also takes place in a cellular compartment that the scavengers cannot reach. From the results obtained with granule-deficient HL-60 cells and cord blood neutrophils, we suggest that the scavenger-insensitive part of the NADPH oxidase activity in normal neutrophils resides in an intracellular compartment identical to or originating from granules. Our results also indicate that specific and azurophil granules have to be in very close contact to allow the generated oxygen metabolites to reach and react with myeloperoxidase.

Citations

Aug 3, 2001·The Journal of Biological Chemistry·B BánfiK H Krause
Apr 24, 2002·Antioxidants & Redox Signaling·Anna Karlsson, Claes Dahlgren
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paige LacyRedwan Moqbel
Sep 22, 2006·Antioxidants & Redox Signaling·Henk-Jan van ManenCees Otto
May 3, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Maria DahlbergCharlotta Movitz
Apr 7, 2016·Annual Review of Biochemistry·Christine C WinterbournMark B Hampton
May 22, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claes DahlgrenJohan Bylund
Apr 23, 2004·The Journal of Biological Chemistry·Glenn E BrownMichael B Yaffe
Dec 24, 2013·Antioxidants & Redox Signaling·Malin HultqvistRikard Holmdahl
Aug 14, 2012·Antioxidants & Redox Signaling·Christine C Winterbourn, Anthony J Kettle
Aug 15, 1998·Annals of the New York Academy of Sciences·A J Verhoeven
Jun 22, 2000·Luminescence : the Journal of Biological and Chemical Luminescence·M Y MagrissoP G Bochev
Jul 29, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·J FäldtC Dahlgren
Jul 11, 2001·Inhalation Toxicology·M AzadnivM W Frampton
Jul 4, 2008·Histochemistry and Cell Biology·John M Robinson
Mar 18, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·Avraham Ashkenazi, Robert S Marks
Apr 23, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Simone B BayerChristine C Winterbourn
May 16, 2017·Antioxidants·Rodrigo Prieto-Bermejo, Angel Hernández-Hernández
Sep 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jasmin FettelThorsten J Maier
Mar 29, 2003·Physiological Reviews·Thomas E Decoursey
Jan 24, 2007·Physiological Reviews·Karen Bedard, Karl-Heinz Krause
Jun 30, 2006·Redox Report : Communications in Free Radical Research·Viera JancinováDagmar Holománová
Mar 12, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Eric Diego BarioniSandra Helena Poliselli Farsky
Jun 15, 2011·Pediatric Blood & Cancer·Andrzej RochowskiBlanche P Alter
Jan 5, 2000·Journal of Immunological Methods·C Dahlgren, A Karlsson
Feb 18, 2016·Analytical Chemistry·Ruiqin HeHong-Yuan Chen
Nov 5, 2014·Analytical Chemistry·Lihong ZhuangDechen Jiang
Aug 2, 2018·Analytical Chemistry·Mingchen XuHong-Yuan Chen

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