Phoresis and Enhanced Diffusion Compete in Enzyme Chemotaxis

Nano Letters
Jaime Agudo-CanalejoRamin Golestanian

Abstract

Chemotaxis of enzymes in response to gradients in the concentration of their substrate has been widely reported in recent experiments, but a basic understanding of the process is still lacking. Here, we develop a microscopic theory for chemotaxis that is valid for enzymes and other small molecules. Our theory includes both nonspecific interactions between enzyme and substrate as well as complex formation through specific binding between the enzyme and the substrate. We find that two distinct mechanisms contribute to enzyme chemotaxis: a diffusiophoretic mechanism due to the nonspecific interactions and a new type of mechanism due to binding-induced changes in the diffusion coefficient of the enzyme. The latter chemotactic mechanism points toward lower substrate concentration if the substrate enhances enzyme diffusion and toward higher substrate concentration if the substrate inhibits enzyme diffusion. For a typical enzyme, attractive phoresis and binding-induced enhanced diffusion will compete against each other. We find that phoresis dominates above a critical substrate concentration, whereas binding-induced enhanced diffusion dominates for low substrate concentration. Our results resolve an apparent contradiction regarding th...Continue Reading

Citations

Nov 3, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ah-Young JeeTsvi Tlusty
Jul 26, 2018·Angewandte Chemie·Krishna Kanti Dey
Oct 28, 2019·Angewandte Chemie·Thomas S C MacDonaldJonathon Edward Beves
Feb 15, 2020·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Gerhard GompperSohan Kale
Jul 8, 2020·Chemistry : a European Journal·Soňa Hermanová, Martin Pumera
Mar 25, 2019·The Journal of Chemical Physics·T Adeleke-LarodoRamin Golestanian
Aug 14, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Motilal MatheshDaniela A Wilson
Aug 7, 2019·Physical Review Letters·Jaime Agudo-Canalejo, Ramin Golestanian
Nov 20, 2019·Nature Nanotechnology·Ambika SomasundarAyusman Sen
Jan 28, 2020·Annual Review of Biophysics·Mudong Feng, Michael K Gilson
May 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jaime Agudo-CanalejoRamin Golestanian
May 9, 2020·Physical Review Letters·Babak Nasouri, Ramin Golestanian
Aug 1, 2020·Science·Huan WangSteve Granick
Nov 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ah-Young JeeSteve Granick

Related Concepts

Metazoa
Chemotaxis
Diffusion
Plasma Protein Binding Capacity
Substrate Specificity
Chemotaxis
Enzyme Activity
Research Study
Urease Activity
Binding (Molecular Function)

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