PMID: 9551090Apr 29, 1998Paper

Phosducin-like protein (PhLP), a regulator of G beta gamma function, interacts with the proteasomal protein SUG1

Biochimica Et Biophysica Acta
S BarhiteM F Miles

Abstract

Phosducin-like protein (PhLP) and phosducin are highly homologous proteins that interact with the beta gamma subunits of guanine nucleotide binding proteins. While phosducin has a well-characterized role in retinal signal transduction, PhLP function remains unclear. To further understand the function of PhLP, we have examined other potential protein:protein interactions with PhLP using the yeast two-hybrid system. PhLP was found to interact with a mouse homologue of the yeast SUG1, a subunit of the 26S proteasome which may also indirectly modulate transcription. This interaction was further confirmed by an in vitro binding assay and co-immunoprecipitation of the two proteins in overexpression studies. Inhibition of proteasome function by lactacystin led to accumulation of high molecular weight, ubiquitin-immunoreactive protein precipitated by PhLP antiserum. We suggest that PhLP/SUG1 interaction may target PhLP for proteasomal degradation.

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Citations

Jan 23, 2013·Molecular Neurobiology·Laura CampelloJosé Martín-Nieto
Apr 22, 2005·Visual Neuroscience·Jing ChenMark W Bitensky
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Joseph N McLaughlinBarry M Willardson
Jun 10, 2000·Molecular and Cellular Biology·P TongaonkarK Madura
Jun 24, 2000·Molecular and Cellular Biology·X Zhu, C M Craft
Feb 28, 2006·Biochemical and Biophysical Research Communications·Takeshi InoueKazuo Sutoh
Jul 31, 2007·Cellular Signalling·Barry M Willardson, Alyson C Howlett
Apr 4, 2006·Developmental Biology·Caroline LacosteMadeleine Erard-Garcia
Feb 24, 2001·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·R Schulz
Dec 4, 2012·Biophysical Journal·Lenka RezabkovaTomas Obsil
Sep 7, 2002·The Journal of Biological Chemistry·Martin ObinAllen Taylor
Apr 6, 2000·The Journal of Biological Chemistry·P L FlanaryH G Dohlman
Jan 20, 2006·The Journal of Biological Chemistry·Christoph KlenkMartin J Lohse
Feb 7, 2001·Biochemical and Biophysical Research Communications·X Zhu, C M Craft

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