Phosphatase inhibition leads to histone deacetylases 1 and 2 phosphorylation and disruption of corepressor interactions.

The Journal of Biological Chemistry
Scott C GalasinskiNatalie G Ahn

Abstract

The regulation of histone deacetylases (HDACs) by phosphorylation was examined by elevating intracellular phosphorylation in cultured cells with the protein phosphatase inhibitor okadaic acid. After fractionation of extracts from treated versus untreated cells, HDAC 1 and 2 eluted in several peaks of deacetylase activity, assayed using mixed acetylated histones or acetylated histone H4 peptide. Stimulation of cells with okadaic acid led to hyperphosphorylation of HDAC 1 and 2 as well as changes in column elution of both enzymes. Hyperphosphorylated HDAC2 was also observed in cells synchronized with nocodazole or taxol, demonstrating regulation of HDAC phosphorylation during mitosis. Phosphorylated HDAC1 and 2 showed a gel mobility retardation that correlated with a small but significant increase in activity, both of which were reversed upon phosphatase treatment in vitro. However, the most pronounced effect of HDAC phosphorylation was to disrupt protein complex formation between HDAC1 and 2 as well as complex formation between HDAC1 and corepressors mSin3A and YY1. In contrast, interactions between HDAC1/2 and RbAp46/48 were unaffected by okadaic acid. These results establish a novel link between HDAC phosphorylation and the co...Continue Reading

References

Apr 6, 1992·Biochimica Et Biophysica Acta·M J Hendzel, J R Davie
Mar 1, 1990·Trends in Biochemical Sciences·P CohenY Tsukitani
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·W M YangE Seto
Sep 1, 1997·Molecular and Cellular Biology·S BartlC Seiser
Mar 7, 1998·The Journal of Biological Chemistry·R Nicol, E Stavnezer
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·S EmilianiE Verdin
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·C A HassigS L Schreiber
Oct 24, 1998·The Journal of Biological Chemistry·Y ZengE Seto
Apr 17, 1999·The Journal of Biological Chemistry·W FischleE Verdin
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·C M GrozingerS L Schreiber
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·R I Yarden, L C Brody
May 14, 1999·Trends in Biochemical Sciences·A Brehm, T Kouzarides
Jun 5, 1999·The EMBO Journal·J KoipallyK Georgopoulos
Jul 20, 1999·Molecular and Cellular Biology·A DoetzlhoferC Seiser
Aug 10, 1999·Seminars in Cell & Developmental Biology·C A Johnson, B M Turner
Dec 22, 1999·The Journal of Biological Chemistry·D WottonJ Massagué
Mar 1, 2000·Trends in Biochemical Sciences·H H Ng, A Bird
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·J LuE N Olson
Jun 28, 2000·Proceedings of the National Academy of Sciences of the United States of America·C M Grozinger, S L Schreiber
Aug 25, 2000·Molecular and Cellular Biology·A H WangX J Yang
May 1, 2001·Biochemical and Biophysical Research Communications·R CaiD Cohen
Aug 18, 2001·Molecular and Cellular Biology·T A McKinseyE N Olson
Oct 17, 2001·The Journal of Biological Chemistry·M K PflumS L Schreiber
Nov 16, 2001·The Journal of Biological Chemistry·Scott C GalasinskiNatalie G Ahn

❮ Previous
Next ❯

Citations

Mar 25, 2008·Cellular and Molecular Life Sciences : CMLS·D P DowlingD W Christianson
Nov 6, 2010·Planta·Ying-Dong BiBai-Chen Wang
Apr 6, 2004·Molecular and Cellular Endocrinology·Maren EckeyAria Baniahmad
Dec 11, 2002·Current Opinion in Cell Biology·Timothy A McKinseyEric N Olson
Apr 4, 2003·Current Opinion in Genetics & Development·Xiang-Jiao Yang, Edward Seto
Feb 28, 2007·Cell Research·Paola GallinariChristian Steinkühler
Oct 20, 2004·The Journal of Biological Chemistry·Brian A Jonas, Martin L Privalsky
Nov 30, 2007·Molecular Biology of the Cell·Gabriela ParoniClaudio Brancolini
Aug 10, 2011·Respirology : Official Journal of the Asian Pacific Society of Respirology·Seiichiro Sakao, Koichiro Tatsumi
Jul 28, 2010·Journal of Virology·Matthew S WaltersSaul Silverstein
Jan 1, 2004·Molecular and Cellular Biology·Heehyoung LeeEdward Seto
Feb 24, 2004·Annual Review of Physiology·Martin L Privalsky
Jan 5, 2011·Journal of Biomedicine & Biotechnology·Chiara V Segré, Susanna Chiocca
Jan 24, 2009·American Journal of Respiratory and Critical Care Medicine·John A MarwickAlberto Papi
Mar 31, 2007·American Journal of Respiratory Cell and Molecular Biology·Dongsun CaoJames M Samet
Oct 18, 2008·American Journal of Respiratory Cell and Molecular Biology·David AdenugaIrfan Rahman
Sep 5, 2002·The Journal of Clinical Investigation·Hugh T Spotswood, Bryan M Turner
Oct 7, 2006·BMC Biochemistry·Nayana KamathMary Kay H Pflum
Nov 4, 2004·BMC Biology·Christina Kaiser, Stephen R James
Sep 22, 2010·International Journal of Chronic Obstructive Pulmonary Disease·John A Marwick, Kian Fan Chung
Oct 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alessandro VanniniStefania Di Marco
Nov 4, 2004·Biochemical and Biophysical Research Communications·Eun-Hye HurHyangshuk Rhim
Apr 25, 2013·The Journal of Biological Chemistry·Dilshad H KhanJames R Davie
Jun 18, 2010·Expert Review of Anticancer Therapy·Brahma N SinghShi-Wen Jiang
Mar 12, 2013·Cell Communication & Adhesion·Gaetano CaramoriIan Adcock
Dec 20, 2011·Free Radical Biology & Medicine·Javier EscobarJuan Sastre
Sep 8, 2004·Journal of Cellular Biochemistry·Nilanjan Sengupta, Edward Seto
Jan 30, 2004·International Journal of Cancer. Journal International Du Cancer·Hanako KobayashiSharon E Fleming
Mar 26, 2004·The Prostate·Kalipso HalkidouCraig N Robson
May 2, 2003·Annals of the New York Academy of Sciences·Sam ThiagalingamJose F Ponte

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

© 2022 Meta ULC. All rights reserved