Phosphate depletion diminishes Mg2+ uptake in mouse distal convoluted tubule cells

Kidney International
L J DaiG A Quamme

Abstract

Hypophosphatemia caused by phosphate depletion is associated with renal magnesium wasting. The cellular mechanisms of phosphate depletion were investigated in an immortalized mouse distal convoluted tubule (MDCT) cell line. Intracellular free Mg2+ concentration. [Mg2+]i was determined by microfluorescence. Mg2+ transport was assessed as a function of change in [Mg2+]i with time following placement of Mg(2+)-depleted cells into a buffer containing 1.5 mM magnesium. The uptake rate of Mg2+ into Mg(2+)-depleted cells cultured in normal phosphate, 1.0 mM, was 175 +/- 21 nM/second. Depletion of phosphate in the culture media was associated with a significant decrease in Mg2+ uptake, which was dependent on the degree of phosphate depletion and on the time cultured in phosphate-deficient media. Cells cultured for 16 hours in 0.3 mM and 0 mM phosphate possessed Mg2+ uptake rates of 105 +/- 18 nM/second and 15 +/- 12 nM/second, respectively. Diminished Mg2+ uptake was rapidly induced following placement in low phosphate and was fully reversed following readdition of phosphate to the culture media. The effects of phosphate depletion on Mg2+ uptake was post-translational in nature as fully up-regulated MDCT cells with maximal Mg2+ uptake ...Continue Reading

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Citations

Nov 14, 1997·Kidney International·G A Quamme
Apr 14, 2012·Diabetes Care·Yusuke SakaguchiYoshiharu Tsubakihara
Aug 21, 2007·Clinical Journal of the American Society of Nephrology : CJASN·Phuong-Chi T PhamPhuong-Thu T Pham
Mar 5, 1998·The American Journal of Physiology·L J DaiG A Quamme
Jan 12, 2001·Physiological Reviews·L J DaiG A Quamme
May 14, 2020·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Periklis KatopodisKonstantinos P Katopodis
Jun 7, 2011·Archives of Biochemistry and Biophysics·Andrea M P Romani

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