Phosphate positioning and availability in the starch granule matrix as studied by EPR

Biomacromolecules
Andreas BlennowMaria Labanowska

Abstract

Cu(2+) was introduced as an EPR probe into the starch granules isolated from different starch crop genotypes including transgenically modified potatoes generated for extreme amylose and starch phosphate monoester concentrations. Several discrete copper adducts bound to the starch matrix with different strength was revealed. It was found that phosphate has a significant influence on the type of these species, their number, location in the structure, and strength of binding. Well dispersed Cu(2+) complexes with axial symmetry are formed in the semicrystalline part of the starch linked through O-P- bonds in the phosphorylated starches. In the amorphous part of the starch, freely rotating hexaaqua complexes of Cu(2+) and complexes coupled antiferromagnetically are formed. The amount of the former increases with content of phosphate indicating enhanced binding of water in the granules. The results complement previous experimental data and molecular models for the starch granule with respect to the location and effects of phosphate and crystalline matter.

References

May 20, 1988·Journal of Molecular Biology·A ImbertyV Tran
Mar 8, 2000·International Journal of Biological Macromolecules·R F Tester, S J Debon
Jun 1, 2000·International Journal of Biological Macromolecules·A BlennowB L Møller
Oct 4, 2003·The Biochemical Journal·René MikkelsenAndreas Blennow
Jul 19, 2005·International Journal of Biological Macromolecules·Andreas BlennowPeter Poulsen

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Citations

Feb 13, 2010·Trends in Plant Science·Andreas Blennow, Søren B Engelsen
Mar 16, 2006·Biopolymers·Maria ŁabanowskaJoanna Szymońska
Dec 1, 2011·Food Chemistry·Hanna Staroszczyk
Dec 5, 2013·Carbohydrate Polymers·Maria LabanowskaAleksandra Wesełucha-Birczyńska
Dec 12, 2012·Carbohydrate Polymers·Maria ŁabanowskaPaulina Puch
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Stijn ReyniersJan A Delcour
May 14, 2008·Journal of Agricultural and Food Chemistry·J SzymońskaE Bielańska

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