PMID: 5378381Nov 1, 1969

Phosphate-stimulated breakdown of 5'-methylthioadenosine by rat ventral prostate

The Biochemical Journal
Anthony E Pegg, H G Williams-Ashman

Abstract

A soluble enzyme preparation catalysing the release of adenine from 5'-methylthioadenosine was purified some 30-fold from extracts of the rat ventral prostate. This reaction was completely dependent on addition of inorganic phosphate ions to the assay medium. This absolute requirement for phosphate ions suggests a phosphorolytic cleavage mechanism. After acid treatment, the other product of the reaction appeared to be 5-methylthioribose. The actions of some other well-characterized enzymes of nucleoside metabolism of 5'-methylthioadenosine were also investigated; purified purine nucleoside phosphorylases from calf spleen and human erythrocytes did not attack 5'-methylthioadenosine. The role of 5'-methylthioadenosine in mammalian tissues is discussed.

Citations

Jan 15, 1990·International Journal of Cancer. Journal International Du Cancer·K KanekoI Akaoka
Sep 1, 1994·Somatic Cell and Molecular Genetics·B W PorterfieldM O Diaz
Nov 1, 2018·The Journal of Biological Chemistry·Anthony E Pegg
Sep 1, 1970·Angewandte Chemie·T Y Shen
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·J I Toohey
Mar 1, 1983·British Journal of Haematology·W N Valentine, D E Paglia
Feb 2, 2008·The Biochemical Journal·Joaquín Fernández-IrigoyenFernando J Corrales
Nov 13, 2008·The Journal of Biological Chemistry·Xiaojing WangAnthony E Pegg
Feb 26, 2016·Oncotarget·Gaia BistulfiDominic J Smiraglia
May 25, 2007·The Journal of Biological Chemistry·Shao-Yang KuP L Howell
Nov 1, 1979·European Journal of Biochemistry·M Carteni'-FarinaVincenzo Zappia
Mar 28, 2008·The Journal of Biological Chemistry·Hong WuAlexander N Plotnikov
Oct 18, 2012·Physiological Reviews·Shelly C Lu, José M Mato
Jun 5, 2019·Critical Reviews in Biochemistry and Molecular Biology·Mark R Sullivan, Matthew G Vander Heiden
Oct 9, 2020·Cell Proliferation·Yang OuyangShengrong Sun

Related Concepts

Adenine
Carbon Isotopes
Bos indicus
Erythrocytes
Pedameth
Nucleosides
Orthophosphate
Prostate
Ribose
Spleen

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.