Phosphate stimulates differentiation and mineralization of the chondroprogenitor clone ATDC5

Japanese Journal of Pharmacology
T FujitaM Koida

Abstract

ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 microg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) / PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3 - 30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. The results suggest a regulatory role of Pi in endochondral osteogenesis.

References

May 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·T AtsumiY Ikawa
Jan 1, 1995·International Review of Cytology·R CanceddaP Castagnola
Aug 1, 1997·Journal of Cellular Biochemistry·F BeierM J Lammi
Aug 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C ShukunamiY Hiraki
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·G R BeckE Moran

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Citations

Nov 16, 2010·The Journal of Biological Chemistry·Atsushi SugitaToshiyuki Yoneda
Sep 18, 2010·Cellular and Molecular Life Sciences : CMLS·Solmaz KhoshniatLaurent Beck
Apr 2, 2010·Differentiation; Research in Biological Diversity·Rani RoyAdele L Boskey
Aug 22, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D MagneJ Guicheux
Mar 17, 2009·Journal of Cellular Biochemistry·Tracy A DenisonBarbara D Boyan
Jan 3, 2013·Journal of Cellular Physiology·Corinne E CamalierGeorge R Beck
Apr 14, 2017·PloS One·Mathilde GuibertArnaud Bianchi
Sep 25, 2003·Journal of Cellular Biochemistry·George R Beck
Sep 16, 2018·Nature Reviews. Endocrinology·Sampada Chande, Clemens Bergwitz
Jun 15, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D MagneJ Guicheux
Aug 31, 2017·Biophysics Reviews·Maytê BoleanPietro Ciancaglini
Sep 4, 2012·International Journal of Molecular Medicine·P T NewtonC Farquharson
Jan 16, 2021·Cells·Federica BoraldiDaniela Quaglino

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