Jan 1, 1975

Phosphate uptake in Chlorella pyrenoidosa : II. Effect of pH and of SH reagents

R Jeanjean


The sensitivity of the phosphate transport system to pCMPS after phosphate starvation is dependent on protein synthesis. This fact is related to the development of transport activity at alkaline pH. In non-starved cells, the presence of only one peak of maximal activity for phosphate uptake at neutral pH (at low and high concentration) has been observed. However, in phosphate starved cells, two peaks of maximal activity (at low phosphate concentration) at neutral and alkaline pH are present. In starved cells, pCMPS inhibits more intensely the phosphate transport activity at alkaline pH than at neutral pH. By contrast, NEM inhibits the phosphate transport more strongly at neutral than at alkaline pH. Phosphate uptake at neutral and alkaline pH are sensitive to osmotic shock, but phosphate uptake at alkaline pH is decreased more than at neutral pH. The results could be interpreted either by assuming that the membrane surroundings change during phosphate starvation or that two transport systems are present in starved cells whereas only one transport system exists in non-starved cells.

Mentioned in this Paper

Phosphate Ion Transport
Phosphate Measurement
Protein Biosynthesis
Sulfhydryl Reagents
Chlorella pyrenoidosa

About this Paper

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.