Jan 1, 1975

Phosphate uptake in Chlorella pyrenoidosa : II. Effect of pH and of SH reagents

Biochimie
R Jeanjean

Abstract

The sensitivity of the phosphate transport system to pCMPS after phosphate starvation is dependent on protein synthesis. This fact is related to the development of transport activity at alkaline pH. In non-starved cells, the presence of only one peak of maximal activity for phosphate uptake at neutral pH (at low and high concentration) has been observed. However, in phosphate starved cells, two peaks of maximal activity (at low phosphate concentration) at neutral and alkaline pH are present. In starved cells, pCMPS inhibits more intensely the phosphate transport activity at alkaline pH than at neutral pH. By contrast, NEM inhibits the phosphate transport more strongly at neutral than at alkaline pH. Phosphate uptake at neutral and alkaline pH are sensitive to osmotic shock, but phosphate uptake at alkaline pH is decreased more than at neutral pH. The results could be interpreted either by assuming that the membrane surroundings change during phosphate starvation or that two transport systems are present in starved cells whereas only one transport system exists in non-starved cells.

Mentioned in this Paper

Ethylmaleimide
4-Chloromercuribenzenesulfonate
Uptake
Phosphate Ion Transport
Phosphate Measurement
Protein Biosynthesis
Sulfhydryl Reagents
Orthophosphate
Chloromercuribenzoates
Chlorella pyrenoidosa

About this Paper

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