PMID: 9421199Jan 8, 1998Paper

Phosphatidic acid elicits calcium mobilization and actin polymerization through a tyrosine kinase-dependent process in human neutrophils: a mechanism for induction of chemotaxis

Biochimica Et Biophysica Acta
R A Siddiqui, D English

Abstract

Phospholipids mediate important effects as extracellular messengers in diverse biological systems. We investigated the effects of phosphatidic acid, a biologically active phospholipid potentially involved in the inflammatory process, on calcium mobilization and actin polymerization in human neutrophils and correlated these effects with induction of chemotactic migration. Intermediate-chain length phosphatidic acid (DiC10-PA) induced a biphasic increase in intracellular Ca2+ characterized by a rapid rise commencing immediately upon addition of stimulus followed by a secondary increase which, unlike the initial response, was eliminated by chelation of extracellular Ca2+. Neither of these responses were induced by C10-lysophosphatidic acid or diacylglycerol. The tyrosine kinase inhibitor herbimycin-A (5-10 microg/ml) completely blunted the initial but not the delayed response effected by DiC10-PA. Long-chain phosphatidic acid (DiC18:1) induced only an initial rapid increase in intracellular Ca2+ and this response was similarly markedly attenuated by herbimycin-A. Among several physiologically relevant phospholipids, only phosphatidic acid was able to induce Ca2+ mobilization; phosphatidylcholine, phosphatidylethanolamine, phosphat...Continue Reading

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Citations

Aug 17, 1999·Current Opinion in Genetics & Development·A Forbes, R Lehmann
Jul 30, 1999·Biochimica Et Biophysica Acta·D W WaggonerD N Brindley
Mar 23, 2010·The Journal of Biological Chemistry·Kathleen FrondorfJulian Gomez-Cambronero
Jan 27, 2006·Molecular Biology of the Cell·Shanjin HuangChristopher J Staiger
Jul 14, 2010·TheScientificWorldJournal·Julian Gomez-Cambronero
Apr 17, 2003·Molecular and Cellular Biology·Bong-Hyun AhnDo Sik Min
Jul 30, 1999·Biochimica Et Biophysica Acta·M G Houle, S Bourgoin
Jan 2, 2008·Molecular and Cellular Endocrinology·R Tomashov-MatarR Shalgi
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May 9, 2001·Biochimica Et Biophysica Acta·R A SiddiquiW Stillwell
Jul 30, 1999·Biochimica Et Biophysica Acta·L C McPhailS Sergeant

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