Aug 30, 1975

Phosphatidic acid metabolism, calcium ions and transmitter release from electrically stimulated synaptosomes

Molecular and Cellular Biochemistry
J N Hawthorne, J E Bleasdale


Synaptosomes isolated from guinea pig brain cortex were stimulated electrically in a medium containing [32P]-orthophosphate. The electrical stimulation caused increased labelling of phosphatidic acid in a synaptic vesicle fraction prepared by osmotic shock of the incubated synaptosomes. Electrical stimulation also provokes transmitter release from the synaptosomes. Both increased phosphatidate labelling and transmitter release required calcium ions in the medium. The effects are discussed in relation to earlier work with acetylcholine and the possible involvement of membrane phosphatidic acid in transmitter release by exocytosis.

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Mentioned in this Paper

Cortex Bone Disorders
Tissue Membrane
Adrenal Cortex Diseases
Phosphatidic Acid Metabolic Process
Synaptic Vesicles
Calcium ion
Synaptic Transmission

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