Phosphatidic acid synthesis in Escherichia coli.

Journal of Bacteriology
M Kito, L I Pizer

Abstract

The kinetic properties of acyl-coenzyme A (CoA): l-alpha-glycerol-phosphate trans-acylase (EC 2.3.1.15) from Escherichia coli were studied. At 10 C, a temperature at which the reaction was proportional to time and enzyme concentration, the enzyme had an apparent K(m) of 60 mum for l-alpha-glycerol-phosphate. The curve describing the velocity of the reaction as a function of palmitoyl-CoA concentration was sigmoid but the plot of v(-1) versus [S](-3) gave a straight line. A K(m) of about 11 mum was calculated for palmitoyl-CoA. Adenosine triphosphate specifically inhibited the reaction, being a noncompetitive inhibitor in respect to l-alpha-glycerol phosphate. Inhibition only occurred with high concentrations of palmitoyl-CoA, and maximal inhibition was 60%.

References

Aug 13, 1968·Biochemical and Biophysical Research Communications·M Kito, L I Pizer
Jun 1, 1968·Journal of Virology·M H Furrow, L I Pizer
Jan 1, 1961·Cold Spring Harbor Symposia on Quantitative Biology·H E UMBARGER
Apr 1, 1963·Journal of Molecular Biology·J MONODF JACOB
Sep 1, 1964·Journal of Bacteriology·L I PIZER, M L POTOCHNY

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Citations

May 1, 1975·European Journal of Biochemistry·M KitoS Sawada
Jan 1, 1994·Critical Reviews in Microbiology·M Suutari, S Laakso
Apr 24, 2002·Biochemical and Biophysical Research Communications·Michael Sinensky
May 1, 1971·Journal of Bacteriology·M Sinensky
Mar 1, 1980·Journal of Bacteriology·K YokotaM Kito
Sep 11, 1975·Bacteriological Reviews·J E Cronan, E P Gelmann
Feb 14, 1972·Biochimica Et Biophysica Acta·J E Cronan, P R Vagelos
Oct 1, 1973·Journal of Bacteriology·J P Merlie, L I Pizer
Sep 1, 2008·EcoSal Plus·John E Cronan, Charles O Rock
Jun 1, 1977·Journal of Bacteriology·M D Snider, E P Kennedy
Mar 1, 1972·Journal of Bacteriology·J E Lusk, E P Kennedy
Oct 1, 1981·Archives of Biochemistry and Biophysics·C O RockJ E Cronan

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