Phosphatidylcholine-specific phospholipase C in mitogen-stimulated fibroblasts

Experimental Cell Research
Carlo RamoniFranca Podo

Abstract

To investigate expression, subcellular localization and mechanisms of translocation of phosphatidylcholine-specific phospholipase C (PC-PLC) during the cell proliferative response, biochemical, immunoblotting, and immunofluorescence analyses were performed on quiescent and mitogen-stimulated NIH-3T3 fibroblasts. Platelet-derived growth factor (PDGF), insulin and 12-O-tetradecanoylphorbol-13-acetate induced, in 10-60 min, PC-PLC translocation from a perinuclear cytoplasmic area to the plasma membrane. Following cell exposure to PDGF (60 min), the overall PC-PLC expression increased up to 2-3x, while the enzyme activity increased 5x in total cell lysates, 2x in the plasma membrane, and 4x in the nucleus; moreover, confocal laser scanning microscopy showed a progressive externalization of PC-PLC on the outer plasma membrane surface and its accumulation in the nuclear matrix. Pre-incubation of cells with the PC-PLC inhibitor tricyclodecan-9-yl potassium xanthate (D609), before PDGF-stimulation, not only reduced the enzyme activity in total cell lysates as well as in plasma membrane and nuclear fractions, but also blocked the mechanisms of PC-PLC subcellular redistribution. These effects were associated with a D609-induced long-last...Continue Reading

References

Oct 1, 1992·Trends in Biochemical Sciences·M Liscovitch
Apr 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E A DennisY A Hannun
Sep 21, 1989·Nature·M J Berridge, R F Irvine
Dec 1, 1989·Biochemical Society Transactions·J MoscatM Lopez-Barahona
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F DolbeareJ W Gray
Jan 1, 1981·Carcinogenesis·R A MufsonI B Weinstein
Jan 27, 1995·Cell·N Divecha, R F Irvine
Apr 14, 1994·Biochimica Et Biophysica Acta·J H Exton
Aug 13, 1993·Cell·N DivechaR F Irvine
Jun 13, 1997·The Journal of Biological Chemistry·S G Rhee, Y S Bae
Aug 28, 1998·Cellular Signalling·J Gomez-Cambronero, P Keire
Dec 5, 1998·Biochimica Et Biophysica Acta·J H Exton
Jun 1, 1997·Seminars in Cell & Developmental Biology·N DivechaC D'santos
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·A Momchilova, T Markovska
May 5, 1999·Current Biology : CB·C S D'SantosN Divecha
Jun 22, 2000·Journal of Cellular Biochemistry·R A Ignotz, T Honeyman
May 9, 2001·Biochimica Et Biophysica Acta·L CoccoF A Manzoli
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C RamoniF Podo

❮ Previous
Next ❯

Citations

Oct 24, 2006·Pharmacogenomics·Kristine Glunde, Natalie J Serkova
Sep 11, 2013·Zygote : the Biology of Gametes and Early Embryos·Federico BonillaMarta Inés Bühler
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Rao Muralikrishna AdibhatlaJames Franklin Hatcher
Oct 17, 2008·Molecular Biology of the Cell·Giacomo CascianelliElisabetta Albi
Nov 22, 2011·Neurochemical Research·Rao Muralikrishna AdibhatlaA Gusain
Oct 11, 2005·Apoptosis : an International Journal on Programmed Cell Death·M IturraldeA Anel
Jan 1, 2013·Molecular Neurobiology·Haviryaji S G KalluriRobert J Dempsey
Oct 24, 2012·Progress in Lipid Research·Igor PokotyloJan Martinec
Jul 12, 2011·Trends in Cardiovascular Medicine·HaiYing LiJunYing Miao
Aug 12, 2008·Neurochemistry International·Melina V MateosNorma M Giusto
Apr 7, 2009·Journal of Cellular Biochemistry·Da FuXing Zhong Wu
Nov 18, 2006·European Journal of Immunology·Francesca SpadaroCarlo Ramoni
Jan 20, 2007·International Journal of Cancer. Journal International Du Cancer·Galit EliyahuHadassa Degani
Mar 10, 2011·NMR in Biomedicine·Franca PodoEgidio Iorio
Jul 15, 2015·Cellular and Molecular Life Sciences : CMLS·Thomas Oliver Eichmann, Achim Lass
Apr 14, 2016·Progress in Lipid Research·Sean P ArlauckasE James Delikatny
Mar 3, 2009·Biochimica Et Biophysica Acta·Julie C RobichaudDennis E Vance
Apr 30, 2015·Expert Review of Molecular Diagnostics·Kristine GlundeZaver M Bhujwalla
Oct 15, 2010·Acta Crystallographica. Section D, Biological Crystallography·S G VachieriA K Basak
Jun 23, 2010·Neuroreport·Haviryaji S G Kalluri, Robert J Dempsey
Dec 21, 2010·Biochemistry. Biokhimii︠a︡·N SjaksteT Sjakste
Nov 18, 2011·Nature Reviews. Cancer·Kristine GlundeSabrina M Ronen
Nov 16, 2007·The Biochemical Journal·Elisabetta AlbiMariapia Viola Magni
Jan 8, 2008·The Journal of Biological Chemistry·Carlos MinahkDennis E Vance
Oct 19, 2005·Cancer Research·Egidio IorioFranca Podo
Nov 5, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Kamila PrymasKatarzyna Kwiatkowska
Feb 13, 2015·Journal of Cell Science·Motohide MurateToshihide Kobayashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.