Phosphatidylethanolamine Induces an Antifibrotic Phenotype in Normal Human Lung Fibroblasts and Ameliorates Bleomycin-Induced Lung Fibrosis in Mice

International Journal of Molecular Sciences
Luis G Vazquez-de-LaraRoberto Berra-Romani

Abstract

Lung surfactant is a complex mixture of phospholipids and specific proteins but its role in the pathogenesis of interstitial lung diseases is not established. Herein, we analyzed the effects of three representative phospholipid components, that is, dipalmitoilphosphatidylcoline (DPPC), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE), on collagen expression, apoptosis and Ca2+ signaling in normal human lung fibroblasts (NHLF) and probed their effect in an experimental model of lung fibrosis. Collagen expression was measured with RT-PCR, apoptosis was measured by using either the APOPercentage assay kit (Biocolor Ltd., Northern Ireland, UK) or the Caspase-Glo 3/7 assay (Promega, Madison, WI, USA) and Ca2+ signaling by conventional epifluorescence imaging. The effect in vivo was tested in bleomycin-induced lung fibrosis in mice. DPPC and PG did not affect collagen expression, which was downregulated by PE. Furthermore, PE promoted apoptosis and induced a dose-dependent Ca2+ signal. PE-induced Ca2+ signal and apoptosis were both blocked by phospholipase C, endoplasmic reticulum pump and store-operated Ca2+ entry inhibition. PE-induced decrease in collagen expression was attenuated by blocking phospholipase C. Finally, s...Continue Reading

References

Oct 29, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·L Vázquez de LaraM Selman
Sep 25, 2003·Biochemical Society Transactions·R S Lewis
Jan 8, 2004·Experimental Biology and Medicine·Mark E BostonDavid G Oelberg
Mar 5, 2004·Toxicology and Applied Pharmacology·Reinhold SchmidtAndreas Günther
Nov 3, 2004·Biochimica Et Biophysica Acta·Anthony G Lee
Feb 7, 2007·International Review of Cytology·Ian A Darby, Tim D Hewitson
Mar 28, 2007·Journal of Cellular Physiology·Kenneth R CutroneoH Paul Ehrlich
Aug 21, 2007·Experimental Gerontology·Carmen CalabresiCarlo Vergani
Jan 8, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Cheryl L FattmanLuis A Ortiz
Apr 12, 2008·Proceedings of the American Thoracic Society·Robert M Strieter
Apr 25, 2008·Biochimica Et Biophysica Acta·Yi Y ZuoFred Possmayer
Nov 4, 2008·European Biophysics Journal : EBJ·Albena JordanovaZdravko Lalchev
Mar 5, 2009·Critical Care : the Official Journal of the Critical Care Forum·Yu SunJian Lu
Aug 12, 2009·The International Journal of Biochemistry & Cell Biology·Luke J JanssenMartin R J Kolb
Feb 4, 2010·Allergy·S Harari, A Caminati
Feb 18, 2010·Journal of Cell Science·Tsuyoshi ArakiJeffrey G Williams
Sep 3, 2010·Trends in Biochemical Sciences·Anant B Parekh
Mar 23, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Ryan W LoneyStephen B Hall
Jul 2, 2011·Lancet·Talmadge E KingMoisés Selman
Sep 6, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Anubhav S Kaviratna, R Banerjee
Apr 27, 2013·The International Journal of Biochemistry & Cell Biology·Subhendu MukherjeeLuke J Janssen
Aug 3, 2013·Current Vascular Pharmacology·Ana M GómezAlejandro Domínguez-Rodríguez
May 14, 2014·International Journal of Molecular Medicine·Li XueYanjiong Chen
Aug 1, 2015·PloS One·Alejandro Guzmán-SilvaRoberto Berra-Romani
Jan 29, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Subhendu MukherjeeLuke J Janssen
Sep 28, 2017·Respiratory Medicine·Martin KolbLutz Wollin
Jan 25, 2018·Archives of Disease in Childhood. Fetal and Neonatal Edition·Christian F Poets, Laila Lorenz
May 10, 2018·The New England Journal of Medicine·David J Lederer, Fernando J Martinez

❮ Previous
Next ❯

Citations

Jun 19, 2020·International Journal of Molecular Sciences·Vidyani SuryadevaraViswanathan Natarajan
Aug 8, 2021·International Journal of Molecular Sciences·Yair Romero, Arnoldo Aquino-Gálvez

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
PCRs
Assay

Software Mentioned

multcomp
R
ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.