Phosphatidylinositol 3-kinase improves the efficiency of positive selection.

International Immunology
Susannah D Barbee, Jose Alberola-Ila

Abstract

We have generated transgenic mice expressing the amino-terminal fragment of the phosphatidylinositol 3-kinase (PI3K) catalytic subunit (p110ABD) in thymocytes. Expression of p110ABD results in constitutive activation of PI3K and in significant increases in the numbers of mature, single-positive thymocytes. We previously reported that the increase in mature cells was in part due to a defect in thymic emigration. In this study we identify another component to this phenotype. Expression of p110ABD results in an enhancement of positive selection, without alterations in thymocyte lifespan or negative selection. Since PI3K can affect activation of Btk, which in turn potentiates calcium fluxes, during B cell development, our results suggest that PI3K could play a role in the regulation of Itk kinases in T cells, and that both cell types share a common signaling network to modulate calcium responses downstream of their antigen receptor.

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Citations

Nov 14, 2007·The Journal of Experimental Medicine·Diana I AlbuDorina Avram
Mar 21, 2007·Biochemical Society Transactions·D T PattonK Okkenhaug
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jens-Peter Holst LauritsenDavid L Wiest
Jun 3, 2021·Genes·Guy WerlenEstela Jacinto
Aug 19, 2010·Science Signaling·Elisabeth FayardGeorg A Holländer

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