PMID: 9166412Jun 2, 1997Paper

Phosphatidylinositol 3-kinase is a negative regulator of cellular differentiation

The Journal of Cell Biology
A PtasznikA Hayek

Abstract

Phosphatidylinositol 3-kinase (PI3K) has been shown to be an important mediator of intracellular signal transduction in mammalian cells. We show here, for the first time, that the blockade of PI3K activity in human fetal undifferentiated cells induced morphological and functional endocrine differentiation. This was associated with an increase in mRNA levels of insulin, glucagon, and somatostatin, as well as an increase in the insulin protein content and secretion in response to secretagogues. Blockade of PI3K also increased the proportion of pluripotent precursor cells coexpressing multiple hormones and the total number of terminally differentiated cells originating from these precursor cells. We examined whether any of the recently described modulators of endocrine differentiation could participate in regulating PI3K activity in fetal islet cells. The activity of PI3K was inversely correlated with the hepatocyte growth factor/scatter factor-induced downregulation or nicotinamideinduced upregulation of islet-specific gene expression, giving support to the role of PI3K, as a negative regulator of endocrine differentiation. In conclusion, our results provide a mechanism for the regulation of hormone-specific gene expression durin...Continue Reading

References

Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·N InagakiH Imura
Feb 1, 1992·The Journal of Clinical Investigation·L KoranyiM A Permutt
Jan 25, 1991·Cell·L C CantleyS Soltoff
Oct 17, 1991·Nature·B J PulvererJ R Woodgett
Feb 1, 1987·The Journal of Clinical Investigation·J PhilippeJ F Habener
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·K TouharaR J Lefkowitz
Jan 1, 1995·Annual Review of Biochemistry·C B Newgard, J D McGarry
Sep 1, 1993·The Journal of Clinical Investigation·T OtonkoskiA Hayek
May 1, 1994·The Journal of Clinical Endocrinology and Metabolism·G M BeattieA Hayek
Aug 31, 1993·Biochemical and Biophysical Research Communications·S S SinghV P Chauhan
Apr 1, 1996·Current Opinion in Cell Biology·C L Carpenter, L C Cantley

❮ Previous
Next ❯

Citations

Dec 4, 2003·Epilepsy Research·Seung-Ki KimByung-Kyu Cho
Jul 11, 2002·Neurochemistry International·Malgorzata ChalimoniukJoanna B Strosznajder
Aug 15, 2000·Molecular and Cellular Endocrinology·J PalgiT Otonkoski
Sep 5, 2002·The Journal of Experimental Medicine·Andrzej PtasznikAlan M Gewirtz
Mar 19, 2009·The Review of Diabetic Studies : RDS·Chee Gee Liew, Peter W Andrews
Jul 13, 2000·Biochemical and Biophysical Research Communications·Q WangB M Evers
Nov 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yuichi HoriSeung K Kim
Mar 16, 2004·Pediatric Diabetes·Rohan K HumphreyAlberto Hayek
Dec 20, 2014·International Journal of Molecular Sciences·S Suresh KumarAkon Higuchi
Mar 15, 2008·Biochemical and Biophysical Research Communications·Hiroaki WatanabeB Mark Evers
Dec 17, 2004·Pediatric Diabetes·Jeremy J Heit, Seung K Kim
Dec 17, 2004·Pediatric Diabetes·Vijayakumar Ramiya, Desmond Schatz
May 6, 2004·Seminars in Cell & Developmental Biology·Alan Colman
Aug 29, 2006·Biochemical Pharmacology·Liaman K MamedovaKenneth A Jacobson
Oct 11, 2005·The International Journal of Biochemistry & Cell Biology·Jinming LiRennian Wang
Jan 5, 2002·Differentiation; Research in Biological Diversity·B Soria
Mar 1, 2005·Differentiation; Research in Biological Diversity·Stéphane DurualJean-Claude Cuber
Jan 18, 2006·Biochemical and Biophysical Research Communications·Xiuwu ZhangTong H Lee
Jun 27, 2006·Bioorganic & Medicinal Chemistry·Enrique RocheBernat Soria
Dec 28, 2002·Diabetes·Latif RachdiRaphael Scharfmann
Jan 6, 2009·Gastroenterology·Lynda ElghaziErnesto Bernal-Mizrachi
May 14, 2004·American Journal of Physiology. Endocrinology and Metabolism·James F List, Joel F Habener
Oct 6, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Emilie GrenierEmile Levy
Jul 25, 2007·Molecular and Cellular Biochemistry·Manon J WinterSergey V Litvinov
Sep 26, 2003·Biochimica Et Biophysica Acta·Lars Neisig MøllerJens Juul Holst

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.