Phosphatidylinositol 4-phosphate accumulates extracellularly upon xylanase treatment in tomato cell suspensions

Plant, Cell & Environment
G GonorazkyLaura de la Canal

Abstract

Various phosphoinositides have been implicated in plant defence signalling. Until now, such molecules have been exclusively related to intracellular signalling. Here, evidence is provided for the detection of extracellular phosphatidylinositol 4-phosphate (PI4P) in tomato cell suspensions. We have analysed and compared the intracellular and extracellular phospholipid profiles of [(32)P(i)]-prelabelled tomato cells, challenged with the fungal elicitor xylanase. These phospholipid patterns were found to be different, being phosphatidylinositol phosphate (PIP) the most abundant phospholipid in the extracellular medium. Moreover, while cells responded with a typical increase in phosphatidic acid and a decrease in intracellular PIP upon xylanase treatment, extracellular PIP level increased in a time- and dose-dependent manner. Using two experimental approaches, the extracellular PIP isoform was identified as PI4P. Addition of PI4P to tomato cell suspensions triggered the same defence responses as those induced by xylanase treatment. These include production of reactive oxygen species, accumulation of defence-related gene transcripts and induction of cell death. We demonstrate that extracellular PI4P is accumulated in xylanase-elicit...Continue Reading

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Citations

Nov 14, 2013·Critical Reviews in Biochemistry and Molecular Biology·Julie Tan, Julie A Brill
Jul 22, 2011·Current Opinion in Plant Biology·Teun Munnik, Erik Nielsen
Nov 6, 2009·Journal of Plant Physiology·Gabriela GonorazkyLaura de la Canal
Sep 16, 2008·Journal of Plant Physiology·Francesca M MittonLaura de la Canal
Aug 9, 2011·Cellular Microbiology·Shiv D Kale, Brett M Tyler
Oct 6, 2014·Current Opinion in Plant Biology·Maya Bar, Adi Avni
Jun 26, 2009·The Biochemical Journal·Hong-Wei XueYu Mei
Apr 21, 2012·Plant Signaling & Behavior·Mariana RegenteLaura de la Canal
Jan 27, 2017·Annual Review of Plant Biology·Katharina GerthIngo Heilmann
Jun 16, 2016·Development·Ingo Heilmann
Sep 4, 2019·Cell Biochemistry and Function·Javier Adrian MoralesEnrique Castano
Feb 18, 2018·Current Opinion in Plant Biology·Brian D Rutter, Roger W Innes
Nov 23, 2018·Progress in Lipid Research·Adiilah Mamode CassimSébastien Mongrand

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