Phosphatidylinositol 4-phosphate negatively regulates chloroplast division in Arabidopsis

The Plant Cell
Kumiko OkazakiHajime Wada

Abstract

Chloroplast division is performed by the constriction of envelope membranes at the division site. Although constriction of a ring-like protein complex has been shown to be involved in chloroplast division, it remains unknown how membrane lipids participate in the process. Here, we show that phosphoinositides with unknown function in envelope membranes are involved in the regulation of chloroplast division in Arabidopsis thaliana. PLASTID DIVISION1 (PDV1) and PDV2 proteins interacted specifically with phosphatidylinositol 4-phosphate (PI4P). Inhibition of phosphatidylinositol 4-kinase (PI4K) decreased the level of PI4P in chloroplasts and accelerated chloroplast division. Knockout of PI4Kβ2 expression or downregulation of PI4Kα1 expression resulted in decreased levels of PI4P in chloroplasts and increased chloroplast numbers. PI4Kα1 is the main contributor to PI4P synthesis in chloroplasts, and the effect of PI4K inhibition was largely abolished in the pdv1 mutant. Overexpression of DYNAMIN-RELATED PROTEIN5B (DRP5B), another component of the chloroplast division machinery, which is recruited to chloroplasts by PDV1 and PDV2, enhanced the effect of PI4K inhibition, whereas overexpression of PDV1 and PDV2 had additive effects. The...Continue Reading

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Citations

Jun 1, 2016·Journal of Experimental Botany·Laia ArmengotYvon Jaillais
Jan 27, 2017·Annual Review of Plant Biology·Katharina GerthIngo Heilmann
Jun 16, 2016·Development·Ingo Heilmann
Jun 21, 2019·Scientific Reports·Jasmine TheisMichael Schroda
Apr 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Alexander P HertleKrishna K Niyogi
Apr 2, 2021·Plant Physiology·Gwennogan A Dubois, Yvon Jaillais

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