Phosphatidylinositol responses are involved in the vascular effects of thiamylal and fentanyl

Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie
O ShibataK Sumikawa

Abstract

Although thiobarbiturates potentiate, and fentanyl attenuates peripheral vasoconstriction, the intracellular mechanism involved in this phenomenon is not clear. Because smooth muscle contraction induced by alpha 1-adrenoceptor agonists is mediated by the phosphatidylinositol (PI) response, this study was carried out to clarify if thiamylal and fentanyl affect the norepinephrine-induced PI response in rat aortic slices. Rat aortic slices were incubated in Krebs-Henseleit solution containing 5 mM LiCl, [3H]myo-inositol, and varying concentrations of thiamylal or fentanyl. The Pl response was stimulated by 0.9 microM (ED50) norepinephrine (NE). The [3H]inositol monophosphate (IP1) was separated from [3H]myo-inositol by column chromatography and counted with a liquid scintillation counter. The basal IP1 accumulation was not affected by thiamylal and fentanyl. Norepinephrine-induced IP1 accumulation was potentiated by thiamylal at concentrations of 10 microM and 100 microM. Norepinephrine-induced IP1 accumulation was attenuated by 1 microM and 10 microM fentanyl. The results suggest that thiamylal stimulates the NE-induced PI response, which potentiates the vasoconstriction, and fentanyl attenuates NE-induced PI response, which woul...Continue Reading

References

Sep 1, 1978·Anesthesia and Analgesia·T H StanleyH R Hill
Sep 1, 1978·Anesthesiology·K E Becker
Oct 1, 1975·Anesthesiology·B T Altura, B M Altura
Feb 1, 1982·Anesthesiology·C E RosowJ J Savarese

❮ Previous
Next ❯

Citations

Sep 8, 2001·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·O ShibataK Sumikawa
Mar 2, 2006·European Journal of Anaesthesiology·S GursoyT Kaya
Oct 12, 2000·Acta Anaesthesiologica Scandinavica·A P Klockgether-RadkeG Hellige

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.