Phospho-proteomic analysis of primary human colon epithelial cells during the early Trypanosoma cruzi infection phase

PLoS Neglected Tropical Diseases
Shankar SumanPius N Nde

Abstract

The protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease, causes severe morbidity and mortality in afflicted individuals. About 30% of T. cruzi-infected individuals present with cardiac, gastrointestinal tract, and/or neurological disorders. Megacolon, one of the major pathologies of Chagas disease, is accompanied by gastrointestinal motility disorders. The molecular mechanism of T. cruzi-mediated megacolon in Chagas disease is currently unknown. To decipher the molecular mechanism of T. cruzi-induced alteration in the colon during the early infection phase, we exposed primary human colonic epithelial cells (HCoEpiC) to invasive T. cruzi trypomastigotes at multiple time points to determine changes in the phosphoprotein networks in the cells following infection using proteome profiler Human phospho-kinase arrays. We found significant changes in the phosphorylation pattern that can mediate cellular deregulations in colonic epithelial cells after infection. We detected a significant increase in the levels of phosphorylated heat shock protein (p-HSP) 27 and transcription factors that regulate various cellular functions, including c-Jun and CREB. Our study confirmed significant upregulation of phospho (p-) Akt...Continue Reading

References

Mar 1, 1989·Molecular and Biochemical Parasitology·M F Lima, F Villalta
Aug 1, 1996·Molecular and Cellular Biology·R JohnsonR Wisdom
Jul 20, 1999·Molecular and Cellular Endocrinology·M Frödin, S Gammeltoft
Apr 1, 2000·Journal of the Autonomic Nervous System·R HaggerD Kumar
Oct 23, 2001·The Journal of Biological Chemistry·Silvia Vaena de AvalosBarbara A Burleigh
Mar 2, 2002·The Lancet Infectious Diseases·A Prata
Sep 13, 2002·The Journal of Biological Chemistry·Hiroshi SakamotoTakashi Tsuruo
Apr 8, 2006·International Journal of Cardiology·Paulo Roberto Benchimol Barbosa
Apr 18, 2006·FEBS Letters·Kaneatra J SimmonsFernando Villalta
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marc Bergeron, Martin Olivier
Feb 28, 2007·The American Journal of Surgical Pathology·Guido IantornoVincenzo Villanacci
May 11, 2007·Human Pathology·Alexandre Barcelos Morais da SilveiraDébora D'Avila Reis
Dec 19, 2007·The Journal of Biological Chemistry·Jagadish C GhoshDario C Altieri
Feb 13, 2008·Journal of Cellular and Molecular Medicine·D LanneauC Garrido
Apr 22, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas LembergerDavid Engblom
Nov 19, 2009·Science Signaling·Marina V Chuenkova, Mercio PereiraPerrin
Apr 20, 2010·Lancet·Anis RassiJosé Antonio Marin-Neto
Nov 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andy Y WenLloyd S Miller
Jan 1, 2010·The Open Parasitology Journal·Tatiana C CardenasFernando Villalta
Jul 19, 2011·Mediators of Inflammation·Zenaida Lopez-DeeLinda S Gutierrez
Jan 24, 2012·Matrix Biology : Journal of the International Society for Matrix Biology·Mariya T Sweetwyne, Joanne E Murphy-Ullrich
Apr 13, 2012·Journal of Parasitology Research·Mauricio M RodriguesMaria Bellio
Aug 17, 2012·Virchows Archiv : an International Journal of Pathology·Sheila Jorge AdadAlessandro Adad Jammal
Nov 8, 2012·Frontiers in Immunology·Pius N NdeFernando Villalta
Jan 22, 2013·Cell Host & Microbe·Kacey L CaradonnaBarbara A Burleigh
Feb 24, 2015·Atencion primaria·Carme Roca SaumellUNKNOWN Grupo de consenso Chagas-APS
Jun 20, 2015·PLoS Neglected Tropical Diseases·Juliana Barreto-de-AlbuquerqueJuliana de Meis
Sep 27, 2015·Global Heart·Jeffrey D Stanaway, Gregory Roth
Jan 16, 2016·PLoS Neglected Tropical Diseases·Aniekanabassi N UdokoPius N Nde
May 26, 2016·Experimental Parasitology·A L A Dos-SantosJ R Meyer-Fernandes
May 20, 2017·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J Y OdaE J A Araújo
Nov 4, 2017·Trends in Neurosciences·Carlos A Saura, Jean-René Cardinaux
Nov 9, 2017·Frontiers in Physiology·Keren GrynbergDavid J Nikolic-Paterson

❮ Previous
Next ❯

Citations

Oct 2, 2019·International Journal of Molecular Sciences·Tatiana Araújo SilvaClaudia Magalhães Calvet
Mar 7, 2021·International Journal of Molecular Sciences·Kayla J RayfordPius N Nde
Oct 22, 2021·Frontiers in Cellular and Infection Microbiology·Nubia Carolina Manchola VarónMaria Julia M Alves

❮ Previous
Next ❯

Methods Mentioned

BETA
profiler
confocal microscopy
sandwich immunoassay
X-ray
transgenic
nuclear translocation

Software Mentioned

IPA
Ingenuity Pathway Analysis Path Explorer ( IPA
SPSS
Image J

Related Concepts