PMID: 11341927May 9, 2001Paper

Phosphodiester and phosphorothioate oligonucleotide condensation and preparation of antisense nanoparticles

Biochimica Et Biophysica Acta
M JunghansA Zimmer

Abstract

Protamine is a cationic peptide with a molecular mass of approx. 4000 Da that is able to condense DNA. In the present study it was used to complex antisense oligonucleotides (ODNs) and to form solid particles with initial diameters of 90-150 nm. The reaction was very rapid and occurred by simple mixing of diluted solutions of the polycation with the oligonucleotide. The aggregation was dependent on the oligonucleotide chain length and the protamine/ODN mass ratio. Particle formation required a minimal chain length of nine nucleotides and a mass ratio of 0.5:1. The particle surface charge and the number of particles depended on the mass ratio. With increasing amounts of the peptide, the number of particles and the zeta potential increased. Both negatively and positively charged particles improved the stability of oligonucleotides against DNase I digestion. Above a mass ratio of 2.5:1 no degradation was found. The uptake of unbound rhodamine-labelled ODNs and its complexes with protamine was determined with Vero cells under in vitro cell culture conditions at 37 degrees C and 4 degrees C. At 37 degrees C the cellular uptake increased with increasing mass ratio. The internalized oligonucleotides were localized in the cytoplasm and...Continue Reading

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Citations

Sep 17, 2009·European Radiology·Daniel M GolovkoHeike E Daldrup-Link
Jun 9, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Monika JunghansAndreas Zimmer
May 4, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Isabella SchöllErika Jensen-Jarolim
Feb 10, 2012·Journal of Pharmaceutical and Biomedical Analysis·David Awotwe-OtooRakhi B Shah
Oct 1, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Rui CamposElena E Ferapontova
Jun 5, 2004·Biochemical and Biophysical Research Communications·P MedberryR K DeLong
Apr 19, 2007·Chemistry, an Asian Journal·Hiroyuki IsobeEiichi Nakamura
Feb 16, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vitali VogelDieter Schubert
Mar 12, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Jörg WeyermannAndreas Zimmer
Nov 30, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jörg WeyermannAndreas Zimmer
Oct 19, 2004·International Journal of Pharmaceutics·Saâdia AsgatayMarie Pierre Rols
Aug 7, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Dirk LochmannAndreas Zimmer
May 4, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Norbert DinauerHagen von Briesen
Apr 22, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·B ScheicherA Zimmer
Dec 25, 2012·International Journal of Pharmaceutics·Sara MovassaghianVladimir P Torchilin
Feb 7, 2015·Free Radical Biology & Medicine·Robert van LithGuillermo A Ameer
Jul 9, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gottfried MayerDieter Schubert
Jul 25, 2014·BioMed Research International·Gyati Shilakari AsthanaSuresh Prasad Vyas
Sep 15, 2004·International Journal of Pharmaceutics·Dirk LochmannAndreas Zimmer
Nov 8, 2005·Nucleosides, Nucleotides & Nucleic Acids·Christian R NoeHelmut Brunar
Mar 15, 2005·Journal of Microencapsulation·D LochmannA Zimmer
Jul 16, 2008·Nanotechnology·Jiesheng YeNa Zhang
Jul 14, 2020·Journal of Cellular Physiology·Zhongtao FengJafar Hajavi
Jul 3, 2021·Nanomaterials·Ivana RuseskaAndreas Zimmer
Dec 19, 2002·Journal of the American Chemical Society·Karen McAllisterJoseph M DeSimone
Nov 27, 2002·Biochemistry·Veena VijayanathanT J Thomas

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