Phosphodiesterases in the rat renal vasculature

Journal of Cardiovascular Pharmacology
E K JacksonR K Dubey

Abstract

The objective this investigation was to determine the relative importance of type I, III, and IV phosphodiesterases in the regulation of cyclic adenosine monophosphate (cAMP) in the renal circulation. In the first experimental series, four groups of isolated rat kidneys perfused with Tyrode's solution were stimulated with isoproterenol (3 microM) and then treated with increasing concentrations (from the approximately IC50 to 30 times the approximately IC50 in threefold increments) of one of four phosphodiesterase inhibitors: group 1, 3-isobutyl-1-methylxanthine, a "broad-spectrum" phosphodiesterase inhibitor (10-300 microM); group 2, Ro 20-1724, a selective type IV phosphodiesterase inhibitor (3-100 microM); group 3, 8-methoxymethyl-3-isobutyl-1-methylxanthine, a selective type I phosphodiesterase inhibitor (3-100 microM); and group 4, milrinone, a selective type III phosphodiesterase inhibitor (0.3-10 microM). In the second experimental series, five groups of cultured preglomerular (interlobular and afferent arteriolar) vascular smooth-muscle cells were stimulated with isoproterenol (1 microM) and treated with vehicle or supramaximal concentrations (30 times IC50) of either 3-isobutyl-1-methylxanthine (300 microM), Ro 20-1724 ...Continue Reading

References

Jan 1, 1988·Methods in Enzymology·J N Wells, J R Miller

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Citations

Mar 26, 2003·European Journal of Pharmacology·Neal J ThomasEdwin K Jackson
Jun 9, 2000·Kidney International·N EndlichJ J Helwig
Jul 4, 2003·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Neal J Thomas, Joseph A Carcillo
Jul 31, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Georges SaabAnitha Vijayan
Oct 21, 2005·Basic & Clinical Pharmacology & Toxicology·Ilhami CelikH Handan Akbulut
Sep 18, 2009·International Journal of Experimental Pathology·Rima Tahseldar-RoumiehClaire Lugnier
Sep 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·Thomas E N JonassenNiels V Olsen
Feb 20, 2007·The Journal of Pharmacology and Experimental Therapeutics·Edwin K JacksonZaichuan Mi

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