Abstract
Phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) is believed to play a significant role in supporting nitrogen fixation via anaplerotic CO2 fixation for recycling carbon in nodules. Using the antisense technique, we decreased the expression levels of the nodule-enhanced PEPC gene (Ljpepc1) in a determinate legume plant (Lotus japonicus) in order to look at the influence of the symbiotic phenotype and biochemical parameters. Three independent transgenic L. japonicus plants (designated as Asppc1, Asppc2 and Asppc3) were prepared using a Ljpepc1 DNA fragment which is under the control of the cauliflower mosaic virus 35S promoter. Extensive suppression of the Ljpepc1 transcript in nodules of Asppc plants (T3 homologous plants) was confirmed by RNA gel blot, Western blot and enzyme activity assays. In nodules of Asppc plants, PEPC activity was reduced to about 10% of that of non-transformants and the plants showed typical nitrogen-deficient symptoms without a supply of nitrogen nutrient, and returned to normal growth when nitrate was supplied at 2.5 mM. The acetylene reduction activity per fresh weight of nodules of these Asppc plants decreased by 29% at 35 dai (days after infection). Various enzyme activities and metabolite leve...Continue Reading
References
May 1, 1986·Analytical Biochemistry·T C Ta, K Joy
Dec 1, 1971·Journal of General Microbiology·A R Ferguson, A P Sims
Dec 1, 1971·The Biochemical Journal·W J Broughton, M J Dilworth
Aug 15, 1970·Nature·U K Laemmli
Aug 1, 1996·Plant Physiology·M S KuM Matsuoka
Jul 15, 1997·Archives of Biochemistry and Biophysics·X Q Zhang, R Chollet
Jan 29, 2000·The Plant Journal : for Cell and Molecular Biology·Y UenoK Izui
Feb 9, 2000·Trends in Plant Science·H G Nimmo
Jul 27, 2001·Planta·G A NimmoH G Nimmo
Feb 15, 2002·Molecular Plant-microbe Interactions : MPMI·Masayoshi KawaguchiShoichiro Akao
Jul 1, 1997·Plant Physiology·A. J. GordonC. L. James
Aug 1, 1995·Plant Physiology·X. Q. ZhangR. Chollet
Apr 1, 1993·Plant Physiology·K. A. Schuller, D. Werner
May 15, 2003·Molecular Plant-microbe Interactions : MPMI·Tomomi NakagawaShingo Hata
Feb 24, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Shigeyuki TajimaHiroshi Kouchi
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Raymond CholletMarion H. O'Leary
Mar 9, 2005·Plant & Cell Physiology·Mika NomuraShigeyuki Tajima
May 1, 1985·Plant Physiology·M Morell, L Copeland
Nov 1, 1990·Plant Physiology·K A SchullerW C Plaxton
Citations
Feb 9, 2010·Journal of Biochemistry·Isamu KameshitaShigeyuki Tajima
Oct 10, 2009·Journal of Experimental Botany·Stephanie A FischingerJoachim Schulze
Apr 7, 2010·Journal of Experimental Botany·Stephanie Anastasia Fischinger, Joachim Schulze
Feb 11, 2011·Journal of Experimental Botany·Mariangela N FotelliEmmanouil Flemetakis
Apr 19, 2012·Journal of Experimental Botany·C TalbiM J Delgado
Jun 4, 2010·Plant & Cell Physiology·Hatthaya ArunothayananShigeyuki Tajima
Jul 28, 2010·Plant & Cell Physiology·Hiroshi KouchiMasayoshi Kawaguchi
Jun 9, 2007·Plant Physiology·James WhitePhilip Poole
Jan 1, 2008·Microbes and Environments·Tan Van DaoShigeyuki Tajima
May 27, 2014·PloS One·Mónica SebastianaOctávio S Paulo
Jun 30, 2014·Journal of Plant Physiology·Iker AranjueloGemma Molero
Jan 30, 2007·The Plant Journal : for Cell and Molecular Biology·Wenxin XuRaymond Chollet
Dec 16, 2010·The New Phytologist·Daniel MarinoNicolas Pauly
Jun 21, 2014·The Plant Journal : for Cell and Molecular Biology·Maryam Nasr EsfahaniLam-Son Phan Tran
Nov 26, 2013·Plant Biotechnology Journal·Maryam Nasr EsfahaniLam-Son Tran
Apr 29, 2011·The Biochemical Journal·Brendan O'LearyWilliam C Plaxton
Nov 1, 2017·The Plant Journal : for Cell and Molecular Biology·Hiroko Yamaya-ItoYosuke Umehara
Sep 25, 2020·Plants·Keiichi NomuraMayu Dozono
May 21, 2018·Plant Molecular Biology·Beate ThalHolger Eubel
Feb 1, 2019·Frontiers in Plant Science·Aoi SogawaMika Nomura
May 4, 2021·Environmental Microbiology·Hao ZhangDa-Zhi Wang
Aug 8, 2021·International Journal of Molecular Sciences·Longlong WangDeqiang Duanmu