Phosphoenolpyruvate carboxylase plays a crucial role in limiting nitrogen fixation in Lotus japonicus nodules

Plant & Cell Physiology
Mika NomuraShigeyuki Tajima

Abstract

Phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) is believed to play a significant role in supporting nitrogen fixation via anaplerotic CO2 fixation for recycling carbon in nodules. Using the antisense technique, we decreased the expression levels of the nodule-enhanced PEPC gene (Ljpepc1) in a determinate legume plant (Lotus japonicus) in order to look at the influence of the symbiotic phenotype and biochemical parameters. Three independent transgenic L. japonicus plants (designated as Asppc1, Asppc2 and Asppc3) were prepared using a Ljpepc1 DNA fragment which is under the control of the cauliflower mosaic virus 35S promoter. Extensive suppression of the Ljpepc1 transcript in nodules of Asppc plants (T3 homologous plants) was confirmed by RNA gel blot, Western blot and enzyme activity assays. In nodules of Asppc plants, PEPC activity was reduced to about 10% of that of non-transformants and the plants showed typical nitrogen-deficient symptoms without a supply of nitrogen nutrient, and returned to normal growth when nitrate was supplied at 2.5 mM. The acetylene reduction activity per fresh weight of nodules of these Asppc plants decreased by 29% at 35 dai (days after infection). Various enzyme activities and metabolite leve...Continue Reading

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Citations

Feb 9, 2010·Journal of Biochemistry·Isamu KameshitaShigeyuki Tajima
Oct 10, 2009·Journal of Experimental Botany·Stephanie A FischingerJoachim Schulze
Feb 11, 2011·Journal of Experimental Botany·Mariangela N FotelliEmmanouil Flemetakis
Jul 28, 2010·Plant & Cell Physiology·Hiroshi KouchiMasayoshi Kawaguchi
Jun 9, 2007·Plant Physiology·James WhitePhilip Poole
Jun 30, 2014·Journal of Plant Physiology·Iker AranjueloGemma Molero
Dec 16, 2010·The New Phytologist·Daniel MarinoNicolas Pauly

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