Phosphoenolpyruvate prevents the decline in hepatic ATP and energy charge after ischemia and reperfusion injury in rats

The Journal of Surgical Research
S SaikiM Tanaka

Abstract

Phosphoenolpyruvate (PEP) is a high-energy metabolite in the final step of glycolysis. PEP is converted into pyruvate by pyruvate kinase. One molecule of adenosine triphosphate (ATP) is generated from one molecule of PEP. The aim of this study was to examine the effects of PEP on hepatic energy metabolism at an early phase after ischemia and reperfusion were examined in rats. Male Wistar rats (250-350 g) were divided into two groups; after two 15-min periods of ischemia with 2 min reperfusion in between, either PEP or glucose solution (400 mmol/liter, pH 7.4) was infused into the portal vein (2.5 ml/300 g body wt/5 min). Before and 0, 5, 10, and 30 min after ischemia, arterial blood and liver tissue were collected for analyses. During the two ischemic periods, ATP and total adenine nucleotide (TAN) of the liver decreased from 9.10 +/- 0.50 and 14.06 +/- 0.29 to 0.99 +/- 0.50 and 10.86 +/- 0.42 mmole/g liver, respectively (P < 0.05), while adenosine monophosphate (AMP) increased from 1.18 +/- 0.15 to 8.47 +/- 0.66 mmole/g liver (P < 0. 05). Hepatic energy charge (EC) significantly decreased from 0.78 +/- 0.02 to 0.16 +/- 0.03 (P < 0.05). Serum concentrations of pyruvate and lactate were elevated from 1.18 +/- 0.15 and 18.4 +/- 0...Continue Reading

References

Jan 17, 1986·Science·P W Hochachka
Jan 1, 1985·Scandinavian Journal of Thoracic and Cardiovascular Surgery·J Hultman, G Ronquist
Dec 1, 1974·The Journal of Clinical Investigation·R A KlonerR B Jennings
Jan 1, 1996·American Journal of Surgery·L CicaleseR Stanko

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Citations

Mar 4, 2003·Biological & Pharmaceutical Bulletin·Saeid GolbidiNaotaka Hamasaki
Dec 11, 2013·Metabolic Syndrome and Related Disorders·Arun P PalanisamyKenneth D Chavin
Jan 3, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Leszek DomanskiKazimierz Ciechanowski
Jun 25, 2005·Nutrition·Danny E C van HoornKlaske van Norren
Oct 3, 2006·Acta Cirúrgica Brasileira·Ilka de Faátima Santana Ferreira BoinLuiz Sergio Leonardi

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