Phosphoethanolamine substitution in the lipid A of Escherichia coli O157 : H7 and its association with PmrC

Microbiology
Sang-Hyun KimCarlton L Gyles

Abstract

This study shows that lipid A of Escherichia coli O157 : H7 differs from that of E. coli K-12 in that it has a phosphoform at the C-1 position, which is distinctively modified by a phosphoethanolamine (PEtN) moiety, in addition to the diphosphoryl form. The pmrC gene responsible for the addition of PEtN to the lipid A of E. coli O157 : H7 was inactivated and the changes in lipid A profiles were assessed. The pmrC null mutant still produced PEtN-modified lipid A species, albeit in a reduced amount, indicating that PmrC was not the only enzyme that could be used to add PEtN to lipid A. Natural PEtN substitution was shown to be present in the lipid A of other serotypes of enterohaemorrhagic E. coli and absent from the lipid A of E. coli K-12. However, the cloned pmrC(O157) gene in a high-copy-number plasmid generated a large amount of PEtN-substituted lipid A species in E. coli K-12. The occurrence of PEtN-substituted lipid A species was associated with a slight increase in the MICs of cationic peptide antibiotics, suggesting that the lipid A modification with PEtN would be beneficial for survival of E. coli O157 : H7 in certain environmental niches. However, PEtN substitution in the lipid A profiles was not detected when putative...Continue Reading

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Citations

May 8, 2013·Antimicrobial Agents and Chemotherapy·William F WalkenhorstWilliam C Wimley
Mar 17, 2007·Annual Review of Biochemistry·Christian R H RaetzRussell E Bishop
Jan 12, 2016·Biochimica Et Biophysica Acta·William F Walkenhorst
Dec 1, 2007·Molecular Microbiology·Thierry TouzéM Stephen Trent
Aug 22, 2009·Biochimica Et Biophysica Acta·Sang-Hyun KimKyu-Tae Chang
Sep 27, 2012·Biochimica Et Biophysica Acta·Luce Farine, Peter Bütikofer
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Nov 25, 2020·Infection and Immunity·Crystal Gadishaw-LueDebora Barnett Foster
Feb 26, 2021·Pharmacological Reviews·Sue C NangJian Li

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