Phosphoglycerylethanolamine posttranslational modification of plant eukaryotic elongation factor 1alpha

Plant Physiology
W D RansomW F Boss

Abstract

Eukaryotic elongation factor 1alpha (eEF-1A) is a multifunctional protein. There are three known posttranslational modifications of eEF-1A that could potentially affect its function. Except for phosphorylation, the other posttranslational modifications have not been demonstrated in plants. Using matrix-assisted laser desorption/ionization-mass spectrometry and peptide mass mapping, we show that carrot (Daucus carota L.) eEF-1A contains a phosphoglycerylethanolamine (PGE) posttranslational modification. eEF-1A was the only protein labeled with [14C]ethanolamine in carrot cells and was the predominant ethanolamine-labeled protein in Arabidopsis seedlings and tobacco (Nicotiana tabacum L.) cell cultures. In vivo-labeling studies using [3H]glycerol, [32P]Pi, [14C]myristic acid, and [14C]linoleic acid indicated that the entire phospholipid phosphatidylethanolamine is covalently attached to the protein. The PGE lipid modification did not affect the partitioning of eEF-1A in Triton X-114 or its actin-binding activity in in vitro assays. Our in vitro data indicate that this newly characterized posttranslational modification alone does not affect the function of eEF-1A. Therefore, the PGE lipid modification may work in combination with ...Continue Reading

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D M RobertsG Stacey
Jan 1, 1991·Cell Motility and the Cytoskeleton·S DharmawardhaneJ Condeelis
Jun 26, 1989·Nucleic Acids Research·A R PokalskyC K Shewmaker
Mar 17, 1986·European Journal of Biochemistry·J A LenstraW Möller
May 1, 1985·Molecular and Cellular Biology·W A FonziP S Sypherd
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W R HiattP S Sypherd
Jun 23, 1995·The Journal of Biological Chemistry·B T EdmondsJ Condeelis
Nov 1, 1994·Cell Biology International·D A CollingsR E Williamson
Oct 14, 1994·Science·N ShiinaE Nishida
Jan 1, 1994·Annual Review of Biochemistry·D R JohnsonJ I Gordon
Apr 21, 1993·Biochimica Et Biophysica Acta·J CavalliusW C Merrick
Oct 1, 1996·Plant Molecular Biology·K S Browning

❮ Previous
Next ❯

Citations

May 8, 2004·Trends in Plant Science·Brandon d Moore
Oct 4, 2003·Plant Physiology·Jose A Lopez-ValenzuelaBrian A Larkins
Mar 11, 2000·Plant Physiology·W D Ransom-HodgkinsW F Boss
Jun 1, 2011·The FEBS Journal·Eva GreganovaPeter Bütikofer
Sep 27, 2012·Biochimica Et Biophysica Acta·Luce Farine, Peter Bütikofer
Dec 23, 2009·Molecular and Biochemical Parasitology·Kai Zhang, Stephen M Beverley
Sep 3, 2020·Chembiochem : a European Journal of Chemical Biology·Alejandra A CarrilesFederico Gago
Jul 3, 2021·International Journal of Molecular Sciences·Alberto Mills, Federico Gago

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.