Phosphoinositide 3-kinase dependent inhibition as a broad basis for opponent coding in Mammalian olfactory receptor neurons

PloS One
Kirill UkhanovBarry W Ache

Abstract

Phosphoinositide 3-kinase (PI3K) signaling has been implicated in mediating inhibitory odorant input to mammalian olfactory receptor neurons (ORNs). To better understand the breadth of such inhibition in odor coding, we screened a panel of odorants representing different chemical classes, as well as odorants known to occur in a natural odor object (tomato), for their ability to rapidly activate PI3K-dependent inhibitory signaling. Odorants were screened on dissociated native rat ORNs before and after pre-incubation with the PI3K-isoform specific blockers AS252424 and TGX221. Many different odorants increased their excitatory strength for particular ORNs following PI3K blockade in a manner consistent with activating PI3K-dependent inhibitory signaling in those cells. The PI3K-dependent inhibitory odorants overlapped with conventional excitatory odorants, but did not share the same bias, indicating partial partitioning of the odor space. Finding that PI3K-dependent inhibition can be activated by a wide range of otherwise conventional excitatory odorants strongly implies PI3K-dependent inhibition provides a broad basis for opponent coding in mammalian ORNs.

References

Oct 6, 1998·Annual Review of Biochemistry·D A FrumanL C Cantley
Mar 25, 1999·Cell·B MalnicL B Buck
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Ma, G M Shepherd
Dec 2, 2000·Nature Neuroscience·R C AranedaS Firestein
Jun 8, 2001·Annual Review of Biochemistry·B VanhaesebroeckM D Waterfield
Jan 22, 2002·Nature Neuroscience·Xinmin Zhang, Stuart Firestein
Mar 5, 2002·Human Molecular Genetics·Janet M YoungBarbara J Trask
Dec 4, 2003·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Ivo Weinhold, Gunter Mlynski
Dec 6, 2003·The European Journal of Neuroscience·Patricia Duchamp-ViretMichel A Chaput
Dec 20, 2003·The EMBO Journal·Yuki OkaKazushige Touhara
Apr 8, 2004·Science's STKE : Signal Transduction Knowledge Environment·Stuart Firestein
Nov 4, 2004·Trends in Genetics : TIG·Shou SerizawaHitoshi Sakano
Dec 16, 2004·The Journal of Biological Chemistry·Elena ShirokovaDietmar Krautwurst
Jan 8, 2005·The Journal of Membrane Biology·A B ZhainazarovB W Ache
Feb 3, 2005·Genome Research·Yoav GiladGustavo Glusman
Feb 18, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sayako KatadaKazushige Touhara
Oct 7, 2005·Genome Biology·Pascale QuignonFrancis Galibert
Nov 5, 2005·Neuron·Barry W Ache, Janet M Young
Feb 14, 2006·Science·Stephen A Goff, Harry J Klee
Apr 18, 2006·Cell·Elissa A Hallem, John R Carlson
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·James D BradyR Lane Brown
Nov 15, 2006·Journal of Neurobiology·Daniela PelzC Giovanni Galizia
Mar 7, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jean-Pierre RosparsPatricia Duchamp-Viret
Apr 1, 2008·Nature Methods·Rafi HaddadNoam Sobel
May 1, 2008·The European Journal of Neuroscience·Kimberly J GrossmanNaoum P Issa
Dec 18, 2008·Journal of Experimental Botany·Sandrine MathieuDenise M Tieman
May 13, 2009·The Journal of General Physiology·Hiroko TakeuchiTakashi Kurahashi
Dec 25, 2009·Journal of Neurophysiology·Kirill UkhanovBarry W Ache
Feb 11, 2010·Nature Reviews. Neuroscience·U Benjamin Kaupp
Jun 4, 2010·Chemical Senses·Barry W Ache
Jan 7, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kirill UkhanovBarry W Ache
May 18, 2011·Nucleic Acids Research·Tyler W H BackmanThomas Girke
Jun 24, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kiyomitsu NaraLinda B Buck
Jan 19, 2012·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Luqman R BachtiarEdmund J Crampin

❮ Previous
Next ❯

Citations

Dec 29, 2020·Molecular and Cellular Neurosciences·Elizabeth A CoreyBarry W Ache
Mar 28, 2021·Journal of Cell Science·Kirill UkhanovJeffrey R Martens
Jun 12, 2021·PLoS Computational Biology·Mitchell E GronowitzThomas A Cleland

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis